2nd method
LO + Flow Imaging
Micro Solutions / Life Science
Light-blocking and flow microscopy system FlowCam LO
Light-blocking and flow imaging in one device: particle control in one run
Product overview
- FlowCam + LO
- Yokogawa
- USA
- FlowCam
- FlowCam + LO
Light-blocking and flow imaging in one device: particle control in one run
USP <787> and <788> for GMP-regulated biopharmaceutical processes
FlowCam LO combines Light Obscuration and flow imaging microscopy in one instrument for integrated particle control in biopharmaceuticals.
One launch simultaneously quantifies the light-blocking and imagery of each particle across more than 30 morphological and optical parameters, a combination of techniques that helps distinguish between protein aggregates, silicone oil, air bubbles and foreign particles, reducing sample size and the number of individual measurements.
The light-blocking module determines the particle size according to high-refractive index polystyrene standards, and the flow microscopy module does not depend on the difference in particle and solvent refractive indices and evaluates the shape and optical properties. The results of both methods are compared in VisualSpreadsheet. <787> and <788> and is designed for use in GMP-controlled processes.
Key figures
30+
particle-meter
USP <787>/<788>
correspondence
GMP
field
Applications
- Control of protein aggregates
- Testing of biopharmaceuticals
- Detection of silicone oil and bubbles
- Quality control in GMP processes
Models and configurations
- FlowCam LO
Product details
Combined modules LO and Flow Imaging
one launch, two methods
Device diagram
Integration of light-blocking and visualization
Two methods in one device
- The Light Obscuration module quickly determines the particle size according to polystyrene standards.
- Flow Imaging receives the image and morphology of each particle regardless of the refractive index.
- The results of both methods are compared in a single report VisualSpreadsheet
For regulated laboratories
- Compliance with USP <787> and <788> requirements for particle control in injectable preparations
- Designed for use in GMP-regulated biopharmaceutical processes
Effectiveness of analysis
- More than 30 morphological and optical parameters per particle
- Combining methods reduces sample consumption and test time
- Helps distinguish between protein aggregates, silicone oil, air bubbles and foreign particles
Measurement methods
| Method 1 | Light Obscuration (light-blocking) - according to the standards of high refractive index polystyrene |
|---|---|
| Method 2 | Flow Imaging – Image and morphology of each particle |
| Measured parameters | more than 30: area, length, width, aspect ratio, equivalent diameter, volume, circularity, compactness, perimeter, brightness, intensity, transparency |
Compliance with requirements
| pharmacopoeia | USP <787>, USP <788> |
|---|---|
| Scope of application | GMP-regulated biopharmaceutical processes |
Software
| VisualSpreadsheet | combines light-blocking and image data in a single report |
|---|
Related equipment types
- VisualSpreadsheet
- FlowCam 8000
- Laminar flow cabinets
- Sample filtration systems
Related products
- Heal Force HFsafe 900 — Laminar flow cabinets
Classification
- Immunology
- Flow imaging microscopy of particles and cells
- Equipment of cell technologies and reproductive medicine
- Analysis of cellular suspension
- Cell counters and viability analyzers
- FlowCam + LO