Micro Solutions / Life Science

A set of environments for vitrification and heating Vitrification & Warming Kit

Ultra-fast vitrification and heating of oocytes and embryos, certified by CE as a Class III medical device

VitaVitro kit for vitrification, type of packaging according to the manufacturer's brochure; current design may vary.

Product overview

Vitrification & Warming Kit
VitaVitro
China
Vitrification
Vitrification & Warming Kit

Ultra-fast vitrification and heating of oocytes and embryos, certified by CE as a Class III medical device

Separate sets of vitrification environments (HV1, HV2, HHM) and heating (HW1, HW2, HHM)

VitaVitro Vitrification & Warming Kit is a set of environments for ultrafast vitrification and subsequent heating of oocytes and embryos in the protocols of assisted reproductive technologies.

The kit consists of two agreed sets: an ethylene glycol-based vitrification solution, DMSO and sucrose, and a solution for subsequent cell heating and rehydration. The environments are ready for use and do not require mixing before the protocol. After vitrification in the environment from the kit, the sample is transferred to a carrier (for example, an Open Pulled straw). Straw) and immersed in liquid nitrogen; HW is applied for heating with gradual dilution of the cryoprotector.

The vitrification kit includes solutions HV1 and HV2 of 1.0 ml and a total diluent HHM 1.0 ml; heating kit - HW1 (2 × 1.5 ml), HW2 1.0 ml and HHM 1.8 ml Buffer environments M199-Hepes, without antibiotics; pH finished solutions 7.2-7.6, endotoxin level less than 0.25 EE / ml, survival in the test on mouse embryos at least 80%. MDR is certified as a Class III medical device (BSI certificates, December 2024).

Key figures

CE

MDR, Class III

pH 7,2–7,6

ready-made environments

≥ 80%

mouse-embryo

Applications

Oocyte vitrification
Embryo vitrification and blastocyst
Warming (warming) after vitrification
Assisted reproductive technologies (ART)

Models and configurations

Vitrification & Warming Kit

Composition of the kit

Vitrification kit: solutions HV1, HV2 and common diluent HHM
Heating kit: solutions HW1 (double volume), HW2 and total diluent HHM
Cryoprotectors: ethylene glycol, dimethyl sulfoxide (DMSO), sucrose
Antibiotic-free environment based on M199-Hepes buffer

Quality control

pH of finished media 7.2–7.6
Endotoxin levels less than 0.25 EE/ml
Test on mouse embryos - survival rate of at least 80%

Vitrification kit

HV1	1.0 ml
HV2	1.0 ml
HHM (diluent)	1.0 ml

Heating kit

HW1	1.5 ml × 2
HW2	1.0 ml
HHM (diluent)	1.8 ml

Quality control and composition

Cryoprotectors	ethylene glycol, DMSO, sucrose
Buffering framework	M199-Hepes, without antibiotics
pH	7,2–7,6
Endotoxins	< 0.25 EE/ml
Test on mouse embryos	≥ 80% survival rate

Certification

CE	MDR Certificate, Class III Medical Device (BSI, December 2024)

Related consumables

Open Pulled Straw (OPS)

Straw for loading and vitrification of the sample in the environment of this kit

Vitrification Cooling Box

Boxing for safe operation with liquid nitrogen during vitrification and heating

Related equipment types

Vitrification equipment
Dewar cryoreservoirs and vessels
Micromanipulators for embryology

Classification

Cellular technology and ART
Vitrification equipment
Equipment of cell technologies and reproductive medicine
ART equipment (IVF)
Vitrification media and gamete cryopreservation systems
Vitrification & Warming Kit

Product images

VitaVitro kit for vitrification, type of packaging according to the manufacturer's brochure; current design may vary.

Open manufacturer image →

Vitrification environment bottles from the official VitaVitro brochure.