Oxford Nanopore
Ligation, Rapid and Rapid PCR Barcoding Libraries
Preparing a DNA library in 10 minutes: the choice between maximum yield and speed
Oxford Nanopore Ligas, Rapid and Barcoded Library Preparation Kits offer a choice between maximum output, speed and multiplexing.
Request a quotationLigas sets keep the length of the input fragments and are oriented towards high yield; Rapid chemistry reduces preparation to about 10 minutes; barcoding combines several samples in one cell. The choice of set depends on the type of sample and the depth required.
In the official line: Ligation Sequencing Kit - about 60 minutes and about 1 μg gDNA; Rapid Sequencing Kit - about 10 minutes and about 200 ng gDNA; Rapid PCR Barcoding - about 15 minutes plus PCR and 1-5 ng gDNA. Compatibility is checked according to the pore and chemistry version.
Selection of the task
- Ligas sets retain the length of the input DNA fragments and give the maximum yield
- Rapid chemistry cuts training to about 10 minutes
- Barcoding combines several samples in one flow cell
Throughput
- Ligation Sequencing Kit: about 60 minutes, intake about 1 μg of gDNA (or 100–200 fmol for amplicons)
- Rapid Sequencing Kit: about 10 minutes, input about 200 ng gDNA (or 50 ng for amplicons)
- Rapid PCR Barcoding Kit: About 15 minutes plus PCR, 1–5 ng gDNA input
Compatibility
- Requires flow cells for DNA (MinION, PromethION, Flongle Flow Cell)
- Compatibility is tested by pore version and current chemistry version
Preparation of the library
| Parameter | Ligation Sequencing Kit | Rapid Sequencing Kit | Rapid PCR Barcoding Kit |
|---|---|---|---|
| Time for preparation | 60 minutes | about 10 minutes | about 15 minutes + PCR |
| Input material | ~1 μg of gDNA (100–200 fmol for amplicons) | ~200 ng gDNA (50 ng for amplicons) | 1-5 ng gDNA |
| Length of reading | length | random distribution depending on the length of the fragment | ~2 kb |
| PCR is required | no | no | yes |
| Key advantage | maximum yield | quickest preparation | minimum entry requirements |
Compatibility
| Parameter | Ligation Sequencing Kit | Rapid Sequencing Kit | Rapid PCR Barcoding Kit |
|---|---|---|---|
| Type of flow cell | DNA cells (MinION, PromethION, Flongle) |