Oxford Nanopore
Direct RNA Sequencing Kit Libraries
Direct sequencing of native RNA without reverse transcription and PCR
The Direct RNA Sequencing Kit allows you to read native RNA directly, storing information about the length of the molecule and modifications.
Request a quotationThe method does not require reverse transcription to form a sequence and helps to investigate isoforms, poly(A) tails and modifications in one setting.
Estimated preparation 135 minutes; input - about 300 ng poly(A)+ RNA or 1 μg total RNA. The length of reading corresponds to the length of the original RNA; PCR is not required. The specific article of the set and the compatible cell are fixed according to the current version of the chemistry.
Method
- Sequencing is performed directly from the RNA molecule, without a reverse transcription step.
- PCR amplification is not required
- The length of reading corresponds to the length of the original RNA molecule
Analysis opportunities
- Allows the isoforms of transcripts and poly(A) tails to be examined
- Provides access to base modifications in the composition of the native RNA molecule
Compatibility
- Requires a flow cell for direct RNA (RNA variant MinION, PromethION or Flongle Flow Cell)
Preparation of the library
| Parameter | Direct RNA Sequencing Kit |
|---|---|
| Time for preparation | 135 minutes |
| Input material | ~300 ng poly(A)+ RNA or 1 μg of total RNA |
| PCR is required | no |
| Length of reading | is |
Compatibility
| Parameter | Direct RNA Sequencing Kit |
|---|---|
| Type of flow cell | RNA variant of flow cells (MinION, PromethION, Flongle) |