~1 min
spotless
Micro Solutions / Life Science
FT-IR strain typing system IR Biotyper
Type strains in hours, not days: 30 isolates from culture to result in ~3 hours
Product overview
- IR Biotyper
- Bruker
- Germany
- IR Biotyper
- IR Biotyper
Type strains in hours, not days: 30 isolates from culture to result in ~3 hours
Infrared spectroscopy for epidemiological surveillance and hospital hygiene
FT-IR system for rapid strain typing and support real-time epidemiological control.
IR Biotyper captures the microorganism's infrared molecular fingerprint and classifies isolates by similarity. The solution helps identify clusters, track transmission pathways and conduct routine screening without lengthy molecular procedures.
Measuring a single spot takes about one minute; 30 samples can be obtained in less than three hours; using a reusable silicon wafer at 96 positions; analysis based on FT-IR spectroscopy, including a diagnostically significant region of 900-1,200 cm-1.
Key figures
96
microplate-position
~3h
30 isolates
FT-IR
method
Applications
- Identification of outbreaks of hospital-acquired infections
- Hospital hygiene and infectious control
- Tracking of transmission routes
- Serotyping (e.g. S. pneumoniae)
- Food microbiology
- Epidemiological studies
Models and configurations
- IR Biotyper
Product details
FT-IR spectrometer IR Biotyper
Analysis of 96-position silicon microplates
Simple workflow
suspension - application - drying - measurement
Speed
- One spot is measured in about a minute, the entire microplate is about 90 minutes.
- A batch of ~30 isolates in three repetitions, along with standards, goes from culture to result in about 3 hours.
- Real-time typing allows you to respond to a flash before it spreads
Analytical capabilities
- Resolution is comparable to routine molecular genetic methods
- By default, the “print” region of polysaccharides 900–1200 cm−1 is analyzed; regions of proteins and fatty acids are available
- The IR Tracker function (kNN algorithm) ranks isolates by kinship and highlights matches by color
- Classification by built-in and own models: INS, SVM, random forest
Integration and economy
- Import of MALDI Biotyper Identity and Export of Results to LIMS in CSV format
- Reusable silicon microplates for 96 positions
- It does not require complex bioinformatics and a powerful IT infrastructure.
Measurement
| Method | Fourier Transformation Infrared Spectroscopy (FT-IR) |
|---|---|
| The main spectral region | 900–1200 cm−1 (polysaccharides); additional 1500–1800 cm−1 (proteins) and 2800–3000 cm−1 (fatty acids) |
| Time of measurement | ~1 min per spot; ~90 min per full microplate |
| Productivity of the workflow | up to 30 isolates (3 repetitions) for ~3 hours |
Samples.
| Format | silicon microtiter microplate with 96 spots, reusable |
|---|---|
| Sample preparation | Suspension of pure culture in bottles of IR Biotyper Kit, application and drying |
Software
| Analysis | PCA (2D/3D), dendrograms, distance matrices, IR Tracker |
|---|---|
| Classification | INS, SVM, random forest; built-in classifiers with regular updates |
| Data exchange | Imports from MALDI Biotyper, exports to LIMS (CSV) |
| Status | Not intended for clinical diagnosis |
Consumables
96-Spot silicon microtiter plates
A set of 5 reusable silicon microplates for 96 positions
IR Biotyper Kit
Two bacterial IR test standards (IRTS 1 and IRTS 2) for 5 runs and vials for sample preparation of 50 isolates
Software
Software package IR Biotyper 4.0
Spectrum analysis, classification and visualization of clusters
Related equipment types
- MALDI-TOF System for Identification of Microorganisms
- Microbiological thermostats
- Biosafety cabinets
- Automatic dispensers
Related products
- Bruker MALDI Biotyper sirius — MALDI-TOF System for Identification of Microorganisms
- Jeio Tech IB-05G (60 l) and IB line — Microbiological thermostats
- Heal Force HFsafe 900 — Biosafety cabinets
- AHN Biotechnologie CAPP Bravo — Automatic dispensers
Classification
- Microbiology
- IR typing of strains
- Analytical instruments for biological samples
- Molecular spectroscopy
- IR/FT-IC spectrometers (concretion analysis, identification)
- IR Biotyper