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IR Biotyper 4.0 Software

Bruker

IR Biotyper 4.0 Software

Clusters, dendrograms and classification of isolates in real time - right during the run

The IR Biotyper 4.0 software package turns FT-IR isolate spectra into visual clusters and answers the main question of infection control - is it a single strain?

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The software manages measurements in IR Biotyper, compares spectral profiles by PCA and LDA, and classifies isolates using neural networks and the reference vector method. Results are shown during the run in the color scheme and are available as dendrograms, distance matrices and 2D/3D graphs; MALDI Biotyper identifications are imported, and MALDI Biotyper identifications are imported. The final data is uploaded to LIMS.

The package runs Windows 10 and requires OPUS 8.2 or higher with OPUS/IT, OPUS/MAP and OPUS/SEARCH licenses; it includes one user license; it supports unlimited metadata of isolates for epidemiological studies; it is not intended for clinical diagnosis.

Analysis of data

  • Statistical methods of PCA and LDA for comparison of spectral profiles
  • Presentation of results in the form of dendrograms, distance matrices, 2D and 3D diagrams
  • Selection of spectral area of analysis: polysaccharides, proteins or fatty acids

Classification

  • Trained classifiers based on artificial neural networks and the method of reference vectors
  • The result of the classification is displayed immediately after the measurement of the spot in the green-yellow-red color scheme.
  • Built-in classifiers are regularly updated by the manufacturer

Working with data

  • Unlimited isolate metadata: virulence factors, resistance, location and date of isolation
  • Import of Identity MALDI Biotyper
  • Exporting results to LIMS

Functions

ParameterIR Biotyper 4.0
Statistical analysisPCA, LDA
Controlled classificationArtificial neural networks (ANN), support vector method (SVM)
Visualizationdendrograms, distance matrices, 2D/3D scattering diagrams
Display of resultsduring the run, after each spot, the color coding

Compatibility

ParameterIR Biotyper 4.0
Dashboard.IR Biotyper
Operating systemWindows 10
DependenciesOPUS 8.2 or higher with OPUS/IT, OPUS/MAP, OPUS/SEARCH licenses
Licenses included1 custom
Data exchangeImport of MALDI Biotyper identifications, export to LIMS
StatusNot intended for clinical diagnosis