96/384
spectrochip
Micro Solutions / Life Science
MassARRAY genotyping system
Multiplex MALDI-TOF genotype analysis: up to 384 samples per cycle without sequencing
Product overview
- MassARRAY System
- Agena Bioscience
- USA
- MassARRAY
- MassARRAY Analyzer 4 + Chip Prep Module 96/384
Multiplex MALDI-TOF genotype analysis: up to 384 samples per cycle without sequencing
Chip Prep Module 96 or 384
Agena Bioscience MassARRAY is an open-source platform for targeted genetic analysis based on MALDI-TOF mass spectrometry. It combines high multiplexity, 96 and 384 well formats and a clear workflow for genotyping, oncology, pharmacogenetics and epigenetics research.
After PCR and SAP processing, the primer is single-basic lengthened; the Chip Prep Module automatically conditions the reaction products and transfers them to SpectroCHIP. MassARRAY Analyzer 4 records the differences in product weight by MALDI-TOF, and Typer software converts the spectra into variant results. SNPs and indeles, somatic mutations, translocations, copy number variations and methylation and do not require sequencing of each sample.
The current system includes MassARRAY Analyzer 4, Chip Prep Module in 96 or 384 configuration and an integrated computer. Supports microplates and SpectroCHIP on 96 or 384 samples; load capacity is two microplates and two arrays. A typical detection time is about 100 minutes for a 96-well and about 150 minutes for a 384-well format. The following analysis takes 5-10 minutes. Complex dimensions are 104 × 110 × 68 cm. For most studies, the manufacturer specifies the input of 10-40 ng of DNA to the reaction; the exact requirements depend on the chemistry and panel. The system is intended for research applications if the particular configuration and set do not have a separate diagnostic status.
Key figures
50
position
10-40 ng
DNA response
Applications
- SNP genotyping
- Somatic mutations
- Translocations and variations in the number of copies
- Pharmacogenetics
- Methylation (EpiTYPER)
- Oncological biomarkers
Models and configurations
- MassARRAY System with Chip Prep Module 96
- MassARRAY System with Chip Prep Module 384
Product details
MassARRAY Analyzer 4
basic-type
Chip Prep Module
sample-making
Download SpectroCHIP
matrix-layer
Throughput
- Format 96: processing time ~100 minutes per microplate, manual time ~25 minutes
- 384 format: processing time ~150 minutes per microplate, manual time ~45 minutes
- Chip Prep Module 384 handles up to 6-8 microplates at 384 holes per shift / day
Detection technology
- Single-basic primer elongation after PCR and SAP processing, without fluorescent labels
- MALDI-TOF detects mass differences in MassARRAY Analyzer 4
- Typer software automatically converts spectra into variant results.
Automation and integration
- Continuous loading of samples minimizes downtime between batches
- Built-in storage system maintains temperature of samples for night runs
- Boot capacity – two microplates and two SpectroCHIP arrays simultaneously
Throughput
| SpectroCHIP format | 96 384 |
|---|---|
| Microplate processing time | ~100 minutes ~150 min |
| Manual time. | ~25 minutes ~45 minutes |
| Subsequent data analysis | 5-10 minutes |
| Chip Prep Module bandwidth | — up to 6-8 microplates for 384 holes per shift / day |
System system
| Components | MassARRAY Analyzer 4, Chip Prep Module, integrated computer |
|---|---|
| Load capacity | 2 microplates + 2 SpectroCHIP arrays |
| Dimensions | 104 × 110 × 68 cm |
| Input DNA to response | 10–40 ng (depending on chemistry and panel) |
| Multiplexity to position | up to 50 options |
| Software | Typer (Analysis and Reporting) |
Application
| Status | for research applications (RUO); individual configurations and sets may have diagnostic status |
|---|
Complementations
Chip Prep Module 96
Automatic preparation and transfer of samples to SpectroCHIP 96
Chip Prep Module 384
Automatic preparation and transfer of samples to SpectroCHIP 384, processing up to 8 microplates / day
Related equipment types
- Thermocycles for PCR
- Nucleic acid extraction systems
- Next-generation sequencing
- LIS for molecular diagnostics
Related products
- DLab Scientific HB120-S — Thermocycles for PCR
- BGI DNBSEQ-E25 — Next-generation sequencing
Classification
- Sequencing and genotyping
- MALDI-TOF genotyping
- Equipment for molecular biology and genetics
- Genotyping and Multiplex Platforms
- MALDI-TOF genotyping platform
- MassARRAY System