Agena Bioscience
Reagent kits for MassARRAY: iPLEX, UltraSEEK, EpiTYPER
MassARRAY chemistry set for genotyping from routine variants to mutations with a 0.1% share
MassARRAY reagent kits allow you to customize the platform for a wide range of tasks - from routine genotyping of inherited variants to highly sensitive search for somatic mutations and quantitative analysis of methylation.
Request a quotationiPLEX Pro is designed for multiplex analysis of germinal variants; iPLEX HS increases sensitivity for somatic variants with low allelic lobe; UltraSEEK uses mutant allele enrichment for even more sensitive analysis; EpiTYPER/MassCLEAVE is used after bisulfite conversion for quantitative profiling. Methylation: The reagent sets are presented as a single compatible ruler, and the specific set is selected by sample type, panel type and sensitivity required.
iPLEX Pro supports up to 40-plex analysis and is commonly used for variants with an allelic fraction of 5-10% or higher. iPLEX HS is designed to detect from about 1% VAF, and UltraSEEK - up to 0.1% VAF under validated conditions. The actual detection limit depends on the matrix, quality and quantity of DNA, panel composition and analytical validation. Agena ClearSEEK reagents are also available for current projects; these are included after confirming a compatible panel and an up-to-date catalog number.
iPLEX Pro
- Multiplex up to 40 variants in a reaction; the reaction contains all four nucleotides in equal concentration
- For germinal genotyping, carrier screening, biomarker validation, pharmacogenetics, breeding animal husbandry and strain validation
- Sensitivity 5-10% in the allelic lobe of the variant
iPLEX HS and UltraSEEK
- iPLEX HS uses nucleotides in a modified ratio to mutant allele enrichment: sensitivity ≥1%, up to 15 markers in the reaction
- UltraSEEK detects only a rare/mutant nucleotide: sensitivity ≥0.1%, up to 15 markers in the reaction
- Samples: heterogeneous solid tumors, fine needle aspiration, circulating tumor DNA from plasma
EpiTYPER / MassCLEAVE
- Quantitative analysis of CpG methylation after bisulfite conversion of the sample
- Sensitivity to detect methylation changes - from 5%
Sensitivity and multiplex
| Parameter | iPLEX Pro | iPLEX HS | UltraSEEK | EpiTYPER (MassCLEAVE) |
|---|---|---|---|---|
| Limit of detection | 5–10% | ≥1% | ≥0,1% | 5 % (change in methylation) |
| Markers for reactions | 40 | 15 | 15 | — |
| Principle of reaction | all 4 nucleotides in equal concentration | nucleotides in modified ratio, enrichment of mutant alleles | only rare/mutant nucleotide | Bisulfite conversion + MassCLEAVE cleavage |
Scope of application
| Parameter | iPLEX Pro | iPLEX HS | UltraSEEK | EpiTYPER (MassCLEAVE) |
|---|---|---|---|---|
| Types of specimens | blood, serum, buccal cells, microdissected and freshly frozen tumor cells, hair follicles, WGA DNA | heterogeneous solid tumors, fine needle aspiration, seed mixtures | circulating tumor and extracellular DNA from plasma | — |
| Typical tasks | pharmacogenetics, hereditary diseases, validation of biomarkers, breeding, validation of culture strains | somatic mutations, low-frequency variants, validation of NGS findings, screening for GMOs | oncogenic markers of resistance and progression, non-invasive testing | quantification |