~40
amino acids in analysis
Micro Solutions / Life Science
HPLC-based amino acid analyzer with post-column derivatization
Automatic analysis of about 40 amino acids in protein hydrolysates and biological fluids
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Product overview
- Young In Chromass
- Korea
- Amino Acid Analyzer
- HPLC-based amino acid analyzer with post-column derivatization
Automatic analysis of about 40 amino acids in protein hydrolysates and biological fluids
with post-column derivatization with ninhydrin and the option of fluorescent detection by OPA
The Young In Chromass post-column derivatization amino acid analyzer automatically detects about 40 primary and secondary amino acids in protein hydrolysates, foods and biological fluids.
The system is based on HPLC separation followed by post-column derivatization: standard - ninhydrin with UV / VIS detection, optionally - ortho-phthalene aldehyde (OPA) with fluorescence detection for higher sensitivity. Reproducibility of results.
The device detects about 40 primary and secondary amino acids in the hydrolysates of protein, collagen, peptides, food products, physiological fluids, tissue extracts, feed, suss and broths.
Key figures
nihydrin
UV/Vis derivatization
OPA
fluorescent derivatization (option)
Applications
- Analysis of protein, collagen and peptides hydrolysates
- Analysis of amino acids in food and beverages
- Analysis of physiological fluids and tissue extracts
- Analysis of feed, sods and broths
Models and configurations
- Amino acid analyzer (postcolumn derivatization)
Automation
- Fully automatic cycle of analysis with minimal sample preparation
- Pre-installed methods without the need to prepare part of the reagents
- Standardized procedure increases reproducibility of results between operators
Detection
- UV/Vis detection by reaction with ninhydrin - for primary and secondary amino acids
- Option fluorescence detection by orthophthaldehyde (OPA) to increase sensitivity
Analytical capabilities
| Method | HPLC with post-column derivatization |
|---|---|
| Number of amino acids to be determined | 40 primary and secondary amino acids |
| Derivatization (UV/Vis) | nihydrin |
| Derivatization (fluorescence, option) | ortho-phthalene aldehyde (OPA) |
| Types of specimens | protein hydrolysates, collagen, peptides, food products, physiological fluids, tissue extracts, feed, wort, broths, drinks |
Related equipment types
- HPLC systems
- Autosamplers for HPLC
- Acid hydrolysis systems for samples
- Columns for amino acid analysis
Classification
- Toxicology and omics
- Amino acid analysis
- Analytical instruments for biological samples
- Chromatography
- HPLC and HPLC analytical
- HPLC-based amino acid analyzer with post-column derivatization