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HPLC-based amino acid analyzer with post-column derivatization

Young In Chromass

HPLC-based amino acid analyzer with post-column derivatization

Automatic analysis of about 40 amino acids in protein hydrolysates and biological fluids

The Young In Chromass post-column derivatization amino acid analyzer automatically detects about 40 primary and secondary amino acids in protein hydrolysates, foods and biological fluids.

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The system is based on HPLC separation followed by post-column derivatization: standard - ninhydrin with UV / VIS detection, optionally - ortho-phthalene aldehyde (OPA) with fluorescence detection for higher sensitivity. Reproducibility of results.

The device detects about 40 primary and secondary amino acids in the hydrolysates of protein, collagen, peptides, food products, physiological fluids, tissue extracts, feed, suss and broths.

Automation

  • Fully automatic cycle of analysis with minimal sample preparation
  • Pre-installed methods without the need to prepare part of the reagents
  • Standardized procedure increases reproducibility of results between operators

Detection

  • UV/Vis detection by reaction with ninhydrin - for primary and secondary amino acids
  • Option fluorescence detection by orthophthaldehyde (OPA) to increase sensitivity

Analytical capabilities

ParameterAmino acid analyzer (postcolumn derivatization)
MethodHPLC with post-column derivatization
Number of amino acids to be determined40 primary and secondary amino acids
Derivatization (UV/Vis)nihydrin
Derivatization (fluorescence, option)ortho-phthalene aldehyde (OPA)
Types of specimensprotein hydrolysates, collagen, peptides, food products, physiological fluids, tissue extracts, feed, wort, broths, drinks