<100 nm
nanopipetta
Micro Solutions / Life Science
Nanopipet platform for the delivery of substances to the cell Single Cellome SU10
Precise delivery of substances into a single living cell with a nanopipetta diameter of less than 100 nm
Product overview
- SU10
- Yokogawa
- Japan
- Single Cellome
- SU10
Precise delivery of substances into a single living cell with a nanopipetta diameter of less than 100 nm
under the control of ion current, with electro-osmotic and electrophoretic delivery
Single Cellome SU10 is a nanopipet platform for targeted delivery and selection of substances from a selected living cell with minimal exposure.
The system automatically detects the surface of the cell through an ion current, injects the nanopipetta to a given depth, and transfers the material with electroosmosis and electrophoresis. It supports delivery to the cytoplasm or nucleus, selection from an individual cell, and observation before and after manipulation with a compatible inverted microscope.
Outer diameter of tip less than 100 nm for SU10ACC-NP01. Rough movement of about 50 mm on each axis with a step of adjustment of 0.625 μm; piezo movement of 100 μm/axis with a step of 10 nm and 1 nm during penetration and extraction. voltage range of -10 ... + 10 V, current measurement of -900 ... + 900 nA; working environment 15-35 °C.
Key figures
1 nm
step-step
±900 nA
current-metering
flot
delivery volume - dozens of femtoliters
Applications
- Delivering genes and proteins to the cell
- Delivery of Cas9 RNP
- Address delivery to the cytoplasm or nucleus
- Immunology
- Cellular and molecular biology
Models and configurations
- SU10
Product details
Nanopipet delivery system
tip diameter less than 100 nm
Automatic determination of the cell surface
ion-drop control
Precision and care
- The diameter of the tip of the nanopipetta is less than 100 nm - an order of magnitude less than with microinjection
- Automatic determination of the cell surface by ion current provides accurate penetration to a given depth
- Electrical delivery mechanism replaces pneumatic or hydraulic pressure
Flexibility of application
- Delivery of a wide range of substances – proteins, genes and ribonucleoproteins Cas9 – as opposed to the Agrobacterium method
- Targeted delivery to specific cells, as opposed to random delivery by Particle Gun
- One nanopipet can process up to 50 cells
Automation
- Continuous delivery to multiple cells via communication with the motorized microscope scene via external trigger
- Compatible with upgrade to CSU-W1 SoRa confocal scanner for pre- and post-delivery surveillance
Drives and positioning
| Gross displacement | stroke ~50 mm/axle, XYZ adjustment step 0.625 μm (motor drive) |
|---|---|
| Precise displacement | stroke 100 μm/axis, step setting XYZ 10 nm, penetration and extraction - 1 nm (piezo drive) |
Measuring module
| Voltage generation range | from -10 V to +10 V, 10 mV setup step |
|---|---|
| Current measurement range | from −900 nA to +900 nA (setting range ±9 V) |
Nanopipetka
| Diameter of the tip | less than 100 nm (for SU10ACC-NP01, reference value) |
|---|---|
| Delivery volume | about a dozen femtoliters per cell |
| One nanopipetka resource | 50 cells |
Dimensions, power supply and conditions
| Main controller | 260×99×280 mm, ~2.8 kg |
|---|---|
| Piezocontroller | 236×88×273 mm, ~4.6 kg |
| Drive module | 270×219×245 mm, ~2.2 kg |
| Measuring module | 85×30×43 mm, ~0.1 kg |
| Power supply | 100–120/220–240 V alternate current, 50/60 Hz, no more than 100 VA |
| Working environment | 15–35 °C, 20–70% relative humidity without condensation, height up to 2000 m |
| Compatibility with microscope | Nikon Ti2 inverted microscopes, Olympus IX71/IX73/IX83, Zeiss Axio Observer (microscope not included) |
Consumables
Nanopipetta SU10ACC-NP01
Glass nanopipetka with an outer diameter of the tip less than 100 nm, a resource of up to 50 cells
Upgrade options
CSU-W1 Confocal Scanner (SoRa option)
Cell observation confocal before and after delivery of the substance
Related equipment types
- CSU-W1 confocal scanners
- Inverted microscopes
- Single Cellome SS2000
- Living cell imaging systems
Related products
- Ningbo Sunny Instruments CLSM610 — CSU-W1 confocal scanners
- Ningbo Sunny Instruments EX30 — Inverted microscopes
- Berthold NightSHADE LB 985 — Living cell imaging systems
Classification
- Immunology
- Systems of analysis and manipulation of single cells
- Equipment of cell technologies and reproductive medicine
- ART equipment (IVF)
- Micromanipulation systems and tools for ICSI, hatching and embryo biopsy
- SU10