SICM
cell surface detection
Micro Solutions / Life Science
Subcellular selection system Single Cellome SS2000
Selection of material from a living cell while preserving the spatial and morphological context
Product overview
- SS2000
- Yokogawa
- Japan
- Single Cellome
- SS2000
Selection of material from a living cell while preserving the spatial and morphological context
confocal imaging, incubation on site and communication with SU10 delivery
Single Cellome SS2000 is an automated subcellular selection system that preserves the spatial and morphological context of each selected cell.
SS2000 combines confocal imaging, target recognition and nanopipet selection from a given area of the cell. Built-in incubation supports the viability of the sample, and the resulting material can be sent for genetic or other molecular analysis with reference to the original image.
The workflow includes nanopipetta input, ion current surface detection, penetration, programmable selection and extraction. The system supports time-lapse and selection after delivery of SU10 reagents; specific configuration parameters, consumables and compatible analytical protocols are determined by the project.
Key figures
5 stages
Approach-Detection-Introduction-Selection-Extraction
Time-lapse
time-lapse
Applications
- Subcellular selection of material
- Genetic and molecular analysis of single cells
- Observation before and after delivery of SU10
- Immunology
- Cell biology
Models and configurations
- SS2000
Product details
Confocal selection control
binding
Incubation on site
persistence
Precise selection
- Selection process: approach - determination of the surface by the fall of ion current - introduction - selection - extraction
- Nanopipet selection from a given subcellular region with preservation of positional and morphological information
- Selection conditions (voltage level, time) are adjusted to the task
Viability of the specimen
- Built-in incubator supports cell viability during selection
- Time-lapse surveillance before and after manipulation
Integration
- Compatible with the delivery of substances by the SU10 system – you can select the material after delivery
- Works in conjunction with CellPathfinder's image analysis software
Principle of work
| Technology for determining surface | based on SICM (scanning ion conductive microscopy) on the fall of ion current |
|---|---|
| Process stages | approach, surface definition, introduction at increased speed, selection, extraction |
| Customizable selection parameters | voltage level and selection time |
Visualization and incubation
| Visualization | Confocal microscopy for positioning and control |
|---|---|
| Incubation | built-in incubator to maintain cell viability during selection |
| Observation | time-lapse before and after selection |
Compatibility
| Compatible software | CellPathfinder |
|---|---|
| Communication to SU10 | Material selection after delivery of substances by the SU10 system |
Related equipment types
- Single Cellome SU10
- CellPathfinder
- CSU-W1 confocal scanners
- Inverted microscopes
Related products
- Ningbo Sunny Instruments CLSM610 — CSU-W1 confocal scanners
- Ningbo Sunny Instruments EX30 — Inverted microscopes
Classification
- Immunology
- Systems of analysis and manipulation of single cells
- Equipment of cell technologies and reproductive medicine
- ART equipment (IVF)
- Micromanipulation systems and tools for ICSI, hatching and embryo biopsy
- SS2000