Micro Solutions / Life Science

Flow-flow imaging microscopy system FlowCam 8000

Flow-through imaging of particles from 2 μm to 1 mm with a sample volume of 100 μl

Product overview

FlowCam 8000
Yokogawa
USA
FlowCam
FlowCam 8000

Flow-through imaging of particles from 2 μm to 1 mm with a sample volume of 100 μl

fluorescent start and compatible with automatic ALH sampling

FlowCam 8000 is a flow imaging microscopy system for counting, measuring and morphologically classifying particles directly from their images.

The device registers each particle found in the stream and simultaneously determines the size, shape, type and distribution. Auto wash reduces transfer between samples, and VisualSpreadsheet allows you to filter data, form class libraries and automate subsequent classification.

Particle range 2 μm-1 mm; lenses 2×, 4×, 10× and 20× with appropriate flow cells. CMOS camera 1920×1200, up to 100 frames / s; processing speed from 0.05 ml / min at 20× to 3 ml / min at 4×. Options 8100 and 8400 support automatic shooting and fluorescent start; software calculates more than 40 particle parameters.

Key figures

2 μm-1 mm

particle-size

100 μl

min sample

100 frames/s

shooting-frequency

40+

particle-meter

Applications

Control of subseen particles in biopharmaceuticals
Characterization of protein aggregates
Control of dry APIs and excipients
Lyophilized drugs
Bioprocesses

Models and configurations

FlowCam 8000

Product details

Flow camera with interchangeable lenses

2×, 4×, 10×, 20×

Analysis of particle imagery

morphology of each particle in a stream

Accuracy of measurements

The range of detectable particles from 2 μm to 1 mm with a minimum sample volume of 100 μl
Advanced Threshold Algorithms Correctly Analyze Transparent Particles
CMOS camera 1920×1200 pixels shoots up to 100 frames per second

Flexibility of configuration

Four lenses (2×, 4×, 10×, 20×) with interchangeable flow cells – processing speeds from 0.05 to 3 ml/min
FlowCam 8400 with fluorescent start and autograph or FlowCam 8100 with autoshot only
Three excitation options (488, 532, 633 nm) with dual channel fluorescence detection

Automation and data

Auto washing and cleaning reduce transfer between samples
Compatible with FlowCam ALH automatic sampling system
VisualSpreadsheet calculates more than 40 parameters and builds libraries for autoclassification

Optics and detection

Particle range	2 μm - 1 mm
Lenses and flow cells	20× (~200×, 50μm cell); 10× (~100×, 100μm option); 4× (~40×, 300/600μm option); 2× (~20×, 1 mm cell)
Camera.	CMOS 1920×1200 pixels, monochrome or color
Frequency of shooting	up to 100 frames/s

Sample preparation and fluidics

Speed of processing of the sample	0.05 ml/min at 20x, up to 3 ml/min at 4x
Pump	microsyringe, volumes 0.5/1/5 ml
Method of obtaining data	FlowCam 8400 – FlowCam launch and autograph; FlowCam 8100 – autograph

fluorescence

Excitation options	488, 532, 633 nm
Detection at 488 nm	Ch1 650 nm (long pass), Ch2 525±15 nm (FITC)
Detection at 532 nm	Ch1 650 nm (long pass), Ch2 575±30 nm (phycoerythrin)
Detection at 633 nm	Ch1 700±10 nm (chlorophyll), Ch2 650±10 nm (phycocyanin)

Software

VisualSpreadsheet	over 40 parameters per particle; filtering, sorting, self-classification libraries

Compatible systems

FlowCam ALH

Automatic preparation and submission of up to 288 samples without operator

VisualSpreadsheet Satellite

Offline data analysis on additional computers

Flow cells

Flow cell 100 μm

For a 10x lens at an average particle range

Flow cell 300/600 μm

For a 4x lens at large particles and high processing speed

fluidic

Syringes 0.5/1/5 ml

Selection of flow speed for the task

Related equipment types

FlowCam ALH
VisualSpreadsheet
Centrifuges for sample preparation
Laminar flow cabinets

Classification

Immunology
Flow imaging microscopy of particles and cells
Equipment of cell technologies and reproductive medicine
Analysis of cellular suspension
Cell counters and viability analyzers
FlowCam 8000