4
sCMOS cameras
Micro Solutions / Life Science
High-content analysis system CellVoyager CV8000
High-performance cell screening: up to 4 cameras and 5 lasers, microplate per minute
Product overview
- CV8000
- Yokogawa
- Japan
- CellVoyager
- CV8000
High-performance cell screening: up to 4 cameras and 5 lasers, microplate per minute
with sample incubation, autofocus and optional robot dispenser for long runs
CellVoyager CV8000 is Yokogawa’s automated HCA platform for high-performance imaging and quantitative analysis of living and fixed cell models.
Wide-field confocal optics, up to four cameras, incubation and an optional robot dispenser support rapid multi-channel experiments, long-term time-lapse, 3D models and cell response studies to add reagents.
Up to 4 sCMOS cameras 2000×2000 pixels and up to 5 lasers 405/445/488/561/640 nm; up to 4 colors simultaneously in confocal mode. 6-1536 wells and subject slides microplates; incubation 35–40 °C. Measuring module 1280×895×1450 mm, weight 510 kg; Dry, water-immersion, phase-contrast and long-working-distance lenses are supported.
Key figures
5
lasers simultaneously
1 536
microplate-hole
1 minute
96 holes, 4 colors
Applications
- High-content screening
- 3D cultures and organoids
- Time-lapse of living cells
- Immunology
- Cell biology
- Phenotypic analysis
Models and configurations
- CV8000
Product details
Automated HCA platform
up to 4 sCMOS cameras
Incubation of the sample on stage
35–40 °C, CO2 5%
Throughput
- Up to 4 sCMOS cameras and 5 lasers capture a four-color 96-well microplate in about a minute.
- 6 to 1,536 holes and slide objects – from screening to single samples
- Laser and imaged autofocus support stable focusing in long runs
Working with living cells
- Incubation on stage supports 35–40 °C and a 5% CO2 supply with forced hydration
- Optional robot dispenser with disposable tips adds reagents during the experiment
- Up to 6 automatically switchable lenses, including water-immersion 20×/40×/60×
Analysis and data
- CellPathfinder program includes machine and deep learning, 3D analysis and label-free methods.
- Data is stored in 16-bit TIFF/PNG and CSV, LZW and ZIP compression is supported
- Optional barcode reader and paired workstations for control and analysis
Optics and detection
| Confocal block | Nipkow dual-disc scanner with microlenses, pinhole 50 μm; 25 μm option and autodisc changer |
|---|---|
| Excitation wavelengths | 405/445/488/561/640 nm, up to 5 solid-state lasers (445 nm for FRET CFP/YFP); LED option 365 nm |
| Mode of shooting. | confocal, up to 4 colors at a time; light field and phase contrast 10×/20×; digital phase contrast |
| Camera. | sCMOS, 2000×2000 pixels, field of view 13×13 mm, up to 4 cameras |
| Objectives | dry 2×/4×/10×/20×; water immersion 20×/40×/60×; phase contrast 10×/20×; long working distance 20× |
Microplates and stage
| Format of the sample | multi-well plates 6-1536 holes, subject slide |
|---|---|
| XY scene | travel X 120 mm, Y 80 mm, installation resolution 0.2 μm |
| Z-axis | range of 4 mm from the focal plane, resolution 0.1 μm |
| Autofocus. | laser-image |
| Incubation on stage | 35–40 °C; 5% CO2 supply with forced humidification; multigas unit option |
Automation and data
| Robot dispenser | Optionally, disposable tips 96 or 384 |
|---|---|
| Barcode reader | optionally, 1D or 2D |
| Analysis | CellPathfinder: granularity, neurites, nuclear morphology and translocation, machine and deep learning, 3D analysis |
| Data formats | Images – 16-bit TIFF, PNG; numerical data – CSV and native format |
Dimensions and power supply
| Dimensions of the measuring unit | 1280 × 895 × 1450 mm |
|---|---|
| Mass of the measuring unit | 510 kg |
| Working environment | 15–30 °C, 30–70% of relative humidity, without condensation |
| Power supply | measuring unit – 100–240 V, 50/60 Hz, up to 2 kVA; workstations – up to 1400 W and 1350 W |
Configuration options
Robot dispenser
Automatic dosing of reagents during the experiment, tips 96 or 384
Barcode reader
1D/2D Microplate Identification
Multigas unit
Supply of additional gases for incubation on stage
Disc with pinholes 25 μm and auto changer
Increased contrast for deep samples
LED 365 nm
Additional excitation channel
CellPathfinder software modules
Machine Learning Module
Segmentation and Gateting Based on Classical Algorithms
Deep Learning Module
Segmentation, cell counting, gated and EC50/IC50 based on neural networks
Related equipment types
- Inverted microscopes
- CO2 incubators
- Robotic liquid dispensers
- Workstations for image analysis
Related products
- Ningbo Sunny Instruments EX30 — Inverted microscopes
- BioAir SafeGrow PRO — CO2 incubators
- AHN Biotechnologie CAPP Bravo — Robotic liquid dispensers
Classification
- Immunology
- High-content analysis systems
- Microscopy and imaging systems
- Automated and scanning systems
- CV8000