Micro Solutions / Life Science

Confocal scanner with CSU-W1 rotating disk

Wide field confocal microscopy of living cells: field of view 17×16 mm, up to 200 frames / s

Product overview

CSU-W1
Yokogawa
Japan
Spinning Disk Confocal CSU
CSU-W1

Wide field confocal microscopy of living cells: field of view 17×16 mm, up to 200 frames / s

three camera configurations and the SoRa superresolution option for sparing photography of living cells

Yokogawa CSU-W1 is a wide-field confocal scanner for careful high-speed imaging of living cells, tissues and bulk biological samples.

Nipkow's dual-rotating microlens drive forms a multi-point scan with low phototoxicity and fading. Motorized confocal and light-field switching, one- or two-camera options, and split-view allow you to assemble the system for time-lapse, multi-channel and deep imaging.

Field of view 17×16 mm; scanning frequency 75–200 Hz at 1500–4000 rpm Install up to two discs with pinholes of 50 or 25 μm; excitation 405–647 nm, optionally NIR 685–785 nm; registration 420–850 nm. T1, T2 and T3 versions, SoRa options, Uniformizer and external light path are available.

Key figures

17x16 mm

field

200 Hz

max. scanning frequency

3

configuration T1/T2/T3

150 nm

SoRa resolution (option)

Applications

Visualization of living cells
Immunology
Cell biology
Time-lapse studies
Multicolor fluorescence

Models and configurations

T1 (1 camera)
T2 (2 cameras)
T3 (Split-view)

Product details

Wide-field disk scanner

field of view 17x16 mm

Three configurations of cameras

T1, T2, T3

Speed and care

Nipkow’s Microlensed Double Rotating Disc Reduces Phototoxicity and Fading During Long Shooting
The disk rotation speed of 1500-4000 rpm gives a scanning frequency of 75-200 frames / s
Motorized switching between confocal and light-field channels without changing optics

Flexibility of configuration

Three basic configurations: one camera (T1), two cameras (T2) and split-view with one camera (T3)
Selection of 50 or 25 microns pinhole discs for different contrast and resolution tasks
Optional NIR port for 685-785 nm excitation and less invasive deep imaging

Extensibility

Upgrade to SoRa superresolution (150 nm XY, 320 nm Z) without replacing the base unit
External light port for photoactivation and connection of additional scanners
All functions can be added after installation of the device.

Optical system

Method of scanning	Microlensed two-disc scanning by Nipkow
Disc speed	1,500–4,000 rpm (75–200 fps)
Pinhole discs	50 μm and 25 μm, motorized shift, up to 2 discs simultaneously
Field of vision	17x16 mm
Excitation wavelength	405–647 nm; option NIR 685–785 nm
The wavelength of the registration	420–850 nm

Configuration T1 / T2 / T3

Number of cameras	1 2 1 (split-view)
Filter-wheel	10-position 10-position x2 6-position
Port of camera	C-mount, 0.83x or 1x
Mass of the main block	18 kg 21.5 kg 19 kg
External dimensions	480×327×252 mm 480×476×252 mm 374×425×252 mm

General parameters

External synchronization	TTL, 300-800 Hz
Working environment	15–35 °C, 20–75% without condensation
Power supply	100–240 V alternate current, 50/60 Hz, no more than 250 VA
Controls	RS-232C (compatible with CSU-X1) commands

SoRa (option)

Superresolution method	Optical Reassignment (SoRa)
Optical resolution	XY 150 nm, Z 320 nm
Switching the increase	motorized, 3 positions, less than 5 seconds

Configuration options

SoRa module

Upgrade to superresolution without replacing the base unit

Disc with pinholes 25 μm

Increased contrast for deep samples

NIR port

Excitation of 685–785 nm for less invasive deep imaging

Motorized lens switch 0.83×/1×/2×

Change the magnification without replacing the lens

External light path

Connecting an external scanner to bypass disks

Related equipment types

Inverted microscopes
Laser light sources
sCMOS cameras
Systems for time-lapse shooting

Classification

Immunology
Confocal scanning systems with a rotating disk
Microscopy and imaging systems
Fluorescent microscopes and modules
CSU-W1