Micro Solutions / Life Science

WesternFLOW PRO Maxi blotting kit

The full workflow of large-format Western blotting: from protein separation to chemiPRO detection

Product overview

westernFLOW PRO Maxi
Cleaver Scientific
Great Britain
Cleaver Scientific
westernFLOW PRO Maxi

The full workflow of large-format Western blotting: from protein separation to chemiPRO detection

vertical electrophoresis, semi-dry transfer and documentation system in one set

Cleaver Scientific WesternFLOW PRO Maxi is a complete large-format western blotting workflow kit: vertical electrophoresis, semi-dry transfer, programmable power supply, reagents and chemiluminescent visualization of chemiPRO in one coherent system.

The kit takes the sample from protein separation to membrane registration. The omniPAGE large camera provides PAGE, the semi-dry module transfers proteins to the membrane in 15 to 30 minutes with a small buffer volume, the powerPRO3AMP powers high-current transfer, and the chemiPRO performs chemiluminescent and fluorescent photography. The semi-dry blotter works with Western. Southern and Northern protocols and gels 0.25-10 mm thick The kit includes the necessary consumables and reagents to start the workflow; the specific configuration is subject to confirmation at the time of order.

WesternFlowPROMAXI kit includes large vertical gel tank, semi-dry blotter, power supply, reagents and chemiPRO system. Semi-dry module of the Midi/Maxi format uses a workspace up to 20×20 cm, platinum anode and stainless steel cathode; typical transfer time of 15–30 minutes, working gel thickness of 0.25–10 mm. The powerPRO3AMP supplies 5–300 V, 10–3000 mA and up to 300 W. chemiPRO is equipped with a 4 MP/16 efficient MP cooled to −57 °C CCD camera, a 16-bit ADC, a motorized F1.2 lens and a seven-position filter wheel; a maximum image area of 32.5×24.1 cm.

Key figures

15-30 minutes

half-dry-transfer

0.25-10 mm

gel-thickness

300 watts

PowerPRO3AMP power capacity

16 MP

efficient resolution of chemiPRO

Applications

Western blotting
Southern and North blotting
Membrane transfer of proteins
Chemiluminescent and fluorescent detection

Models and configurations

westernFLOW PRO Maxi

Product details

Full workflow

electrophoresis to detection

Transfer systems

Cleaver blotting options

Half-dry blotter

platinum anode, stainless steel cathode

Composition of the kit

omniPAGE camera for vertical electrophoresis (PAGE)
Semi-dry Midi/Maxi blotter with a workspace of up to 20x20 cm
PowerPRO3AMP power supply for high-current transfer
chemiPRO Documentation System for Chemiluminescent and Fluorescent Photography

Protein transfer

Semi-dry transfer in 15-30 minutes with low buffer consumption
Platinized anode and stainless steel cathode are practically not subject to corrosion
Supports Western, Southern and Northern protocols, gels 0.25-10 mm thick

Detection

Refrigerated CCD camera chemiPRO 4 MP (effectively 16 MP), 16-bit ADC
Maximum image area 32.5×24.1 cm

Transfer (half-dry blotter)

Format	Midi/Maxi, working area up to 20x20 cm
Electrodes	platinum anode, stainless steel cathode
Transfer time	15-30 minutes
Gel thickness	0.25-10 mm

Power (powerPRO3AMP)

Tension.	5-300 V
Current	10–3000 mA
Power	300 watts

Documentation (chemiPRO)

Camera.	cooled CCD 4 MP (effectively 16 MP)
ADC	16 bits, 65,536 gray gradations
Lens/filters	Motorized zoom F1.2, 7-position filter wheel
Image area	max. 32.5 x 24.1 cm

ChemiPRO options in the set

HI-LED RGB/RGBIR module

Expansion to multiplex multiple channels

Retractable UV transilluminator 302 nm

For working with gels (different sizes)

Related equipment types

Gell documentation systems
Cameras for PAag electrophoresis
Power supplies for electrophoresis
Blotting supplies

Classification

PCR and electrophoresis
Western blotting
Equipment for molecular biology and genetics
Electrophoresis and documentation
Systems of vertical electrophoresis and protein transfer (Western blot)
WesternFLOW PRO Maxi blotting kit