Micro Solutions / Life Science

Inserts for cell culture SPLInsertTM SPL

Membrane inserts for transport, migration and barrier studies - pores 0.4, 3.0 and 8.0 μm

Product overview

SPL
Republic of Korea
SPL
culture inserts

Membrane inserts for transport, migration and barrier studies - pores 0.4, 3.0 and 8.0 μm

Hanging and Standing for 6-, 12- and 24-well microplates

SPLInsertTM is a membrane culture insert for reproducible studies of transport, migration, invasion and co-culture of cells.

The suspension and standing structures form separate apical and basolateral compartments, and track membranes with controlled pore size and density are suitable for barrier models, permeability assessment, chemotaxis, angiogenesis and drug transport.

Polyester (PET) or polycarbonate (PC) membranes, pore sizes 0.4; 3.0 and 8.0 μm, nominal thickness of about 10–23 μm Suspension formats: 6/12/24-well, growth area 4.52/0.92/0.33 cm2; standing: 6/24-well, 4.06/0.60 cm2. PS or PET frame depending on the series; sterile options.

Key figures

0.4/3.0/8.0 μm

pore

PC/PET

membrane

6/12/24

microplate

Hanging/Standing

execution

Applications

Research on invasion and cell migration
Transport and permeability studies
Co-culture (co-culture)
Hemotaxis and angiogenesis
Barrier models in vivo
Polarization and differentiation of the epithelium

Models and configurations

6-well Hanging
12-well Hanging
24-well Hanging
6-well Standing
24-well Standing

Product details

SPLInsert Hanging

insertion

SPLInsert Standing

insertion stands on three supports at the bottom of the hole

Membranes

Track membranes with controlled pore size and density: 0.4, 3.0 and 8.0 μm
Polyester (PET) – high chemical resistance and low protein binding
Polycarbonate (PC) – not stained, low background signal
Nominal thickness of the membrane is about 10–23 μm

Formats

Hanging – the insert rests on the edges of the hole
Standing performance (Standing) - the insert stands on three supports at the bottom of the hole, closer to the monolayer
Formats for 6-, 12- and 24-well microplates, including separate inserts without a microplate (Insert Only)

Quality.

Packed in culture plates with treated surface (except Insert Only)
Cytotoxicity, apyrogenicity, absence of RNase/DNAse and DNA confirmed

Geometry

Area of growth	4.52 cm² 0.90 cm2 0.33 cm2 4.06 cm² 0.60 cm2
Insert working volume	1.5–2.5 ml 0.4–1.0 ml 0.2–0.35 ml 2.0–6.0 ml 0.2–0.6 ml
Distance from membrane to bottom of hole	1.20 mm 1.20 mm 1.30 mm 0.91 mm 0.85 mm
Diameter of the membrane	24.00 mm 10.50 mm 6.50 mm 22.74 mm 8.75 mm

Material and pores

Membrane material	Polycarbonate (PC) or polyester (PET)
Pore size	0.4; 3.0 or 8.0 μm
Materials of the framework	polystyrene (PS) or PET, depending on the series
Membrane thickness	10–23 μm

Additional formats

SPLInsert Insert Only

Separate inserts without a complete cultural microplate - installed in the microplate independently

Related equipment types

Cultural microplates
Flow cytometers
Inverted microscopes
CO2 incubators

Classification

Cellular technology and ART
Plastic for cell culture
Consumables and laboratory plastics
Cultural plastic
Scrapers, cell filters, inserts
Inserts for cell culture SPLInsertTM SPL