20 000–30 000
drop
Micro Solutions / Life Science
DropX-2000 Digital PCR System
Droplet digital PCR with generation of up to 30,000 droplets per sample for absolute quantification
Product overview
- DropX-2000
- RainSure
- China
- DropX
- DropX-2000
Droplet digital PCR with generation of up to 30,000 droplets per sample for absolute quantification
two devices – SG-2000 amplifier and DS-2000 biochip scanner – without external compressor
RainSure DropX-2000 is a two-modular drip digital PCR system for absolute quantitative analysis of nucleic acids and detection of rare genetic variants.
The platform combines automated sample preparation, drop generation and PCR amplification in the SG-2000 module with subsequent reading and analysis in the DS-2000 biochip scanner. The operator manually deposits a sample and transfers a closed chip between the two instruments; a separate pressure source and an external amplifier are not required. It separates positive and negative drops and calculates the concentration of nucleic acids according to the distribution of Poisson.
The integrated chip generates 20,000-30,000 uniform droplets in a closed system. The scanner uses four fluorescent channels — FAM, HEX/VIC, ROX and Cy5 — and supports multiplex analysis. The platform is compatible with probe and dye-binding detection methods, stores images of droplets for quality control and allows repeated re-analysis. The manufacturer specifies the applications: absolute quantification, analysis of copy number variations, rare mutations, small amounts of nucleic acids, mRNA/miRNA and control of standards for NGS.
Key figures
4
fluorescent channels
2
instrument: SG-2000 + DS-2000
Applications
- Absolute Quantitative Assessment of Nucleic Acids
- Detection of rare mutations
- Analysis of Copy Number Variations (CNV)
- Analysis of incDNA and small amounts of NDT
- mRNA/miRNA
- Control of standards for NGS
Models and configurations
- DropX-2000
Product details
Sample preparation and amplification module SG-2000
Drop and PCR generation in a closed system
Biochip scanner DS-2000
Reading and analysis of drops on 4 channels
Digital PCR workflow
from the sample to the Poisson concentration calculation
Workflow
- Automated sample preparation, drop generation and PCR amplification are combined in the SG-2000 module.
- Operator manually transfers the closed chip to the DS-2000 scanner – no external pressure source and no separate amplifier required
- Programmatic analysis separates positive and negative drops and calculates concentration by Poisson distribution
Analysis opportunities
- An integrated chip forms 20,000-30,000 uniform droplets in a closed system.
- Four fluorescent channels – FAM, HEX/VIC, ROX and Cy5 – support multiplex analysis.
- Compatibility with probe and dye-binding detection methods
Quality control
- Images of drops are saved for quality control analysis
- Re-analysis of the sample is possible within a week after launch
Platform
| Composition of the system | SG-2000 sample preparation and amplification module + DS-2000 biochip scanner |
|---|---|
| Drop generation | 20,000-30,000 drops per reaction, closed system |
| Fluorescent channels | FAM, HEX/VIC, ROX, Cy5 |
| Detection method | probe and dye-binding methods |
| Calculation of concentration | by the Poisson distribution, automatically |
| Reanalysis of the sample | weekly |
Related equipment types
- Thermocycles for real-time PCR
- Nucleic acid isolation systems
- Laminar boxes for PCR zones
- NGS sequencing
Related products
- DLab Scientific HB120-S — Thermocycles for real-time PCR
- Yokogawa CV8000 — Nucleic acid isolation systems
- Heal Force HFsafe 900 — Laminar boxes for PCR zones
- BGI DNBSEQ-E25 — NGS sequencing
Classification
- PCR and electrophoresis
- Digital PCR
- Equipment for molecular biology and genetics
- Amplification
- Digital PCR Systems (dPCR)
- DropX-2000