10 minutes
quickest preparation
Micro Solutions / Life Science
Ligation, Rapid and Rapid PCR Barcoding Libraries
Preparing a DNA library in 10 minutes: the choice between maximum yield and speed
Product overview
- Ligase, fast (Rapid), barcoding sets
- Oxford Nanopore
- Great Britain
- Library Prep Kits
- Ligase, fast (Rapid), barcoding sets
Preparing a DNA library in 10 minutes: the choice between maximum yield and speed
Ligase, Rapid and Barcoding Chemistry for Native and Amplified DNA
Oxford Nanopore Ligas, Rapid and Barcoded Library Preparation Kits offer a choice between maximum output, speed and multiplexing.
Ligas sets keep the length of the input fragments and are oriented towards high yield; Rapid chemistry reduces preparation to about 10 minutes; barcoding combines several samples in one cell. The choice of set depends on the type of sample and the depth required.
In the official line: Ligation Sequencing Kit - about 60 minutes and about 1 μg gDNA; Rapid Sequencing Kit - about 10 minutes and about 200 ng gDNA; Rapid PCR Barcoding - about 15 minutes plus PCR and 1-5 ng gDNA. Compatibility is checked according to the pore and chemistry version.
Key figures
60 minutes.
ligase set, maximum output
1-5 ng
GDNA for Rapid PCR Barcoding
RUO
research
Applications
- Native DNA sequencing
- Multiplexing of Barcode Samples
- Amplicon sequencing
- Fast preparation of libraries in the field
Models and configurations
- Ligation Sequencing Kit
- Rapid Sequencing Kit
- Rapid PCR Barcoding Kit
Product details
Library training kit
Ligase, Rapid and Barcoding Chemistry
Line of library preparation kits
Comparison of preparation time and input material
Selection of the task
- Ligas sets retain the length of the input DNA fragments and give the maximum yield
- Rapid chemistry cuts training to about 10 minutes
- Barcoding combines several samples in one flow cell
Throughput
- Ligation Sequencing Kit: about 60 minutes, intake about 1 μg of gDNA (or 100–200 fmol for amplicons)
- Rapid Sequencing Kit: about 10 minutes, input about 200 ng gDNA (or 50 ng for amplicons)
- Rapid PCR Barcoding Kit: About 15 minutes plus PCR, 1–5 ng gDNA input
Compatibility
- Requires flow cells for DNA (MinION, PromethION, Flongle Flow Cell)
- Compatibility is tested by pore version and current chemistry version
Preparation of the library
| Time for preparation | 60 minutes about 10 minutes about 15 minutes + PCR |
|---|---|
| Input material | ~1 μg of gDNA (100–200 fmol for amplicons) ~200 ng gDNA (50 ng for amplicons) 1-5 ng gDNA |
| Length of reading | length random distribution depending on the length of the fragment ~2 kb |
| PCR is required | no no yes |
| Key advantage | maximum yield quickest preparation minimum entry requirements |
Compatibility
| Type of flow cell | DNA cells (MinION, PromethION, Flongle) |
|---|
Compatible flow cells
MinION Flow Cell
Flow cell for Minion Mk1D and Gridion devices
PromethION Flow Cell
High-performance flow cell for PromethION devices
Flongle Flow Cell
Small-format cell for small and economical launches
Related equipment types
- Nanopore sequencing
- Flow cells
- Software for analyzing sequencing data
Related products
- BGI DNBSEQ-E25 — Nanopore sequencing
- Cytek Cytek Aurora Evo — Flow cells
Classification
- Sequencing and genotyping
- Libraries training kits
- Equipment for molecular biology and genetics
- Reagents and kits for molecular diagnostics
- NGS library preparation and enrichment kits
- Ligation, Rapid and Rapid PCR Barcoding Libraries