4
fluorescence channel (max.)
Micro Solutions / Life Science
EX30 fluorescent platform for immunofluorescence and FISH with fast transition to light field
EX30 fluorescent platform for immunofluorescence and FISH with fast transition to light field
Single or multichannel LED fluorescence with a trinocular head
Sunny EX30FL is a fluorescent configuration of the EX30 universal microscopic platform for immunofluorescence, FISH, clinical laboratory diagnostics and biomedical research. The modular system combines passing light with single or multichannel fluorescence and allows you to tailor optics and filters for specific fluorochromes.
EX30FL supports fast switching between light field and fluorescent observation. In a single-channel LED configuration, each interchangeable module combines the excitation source and the corresponding filter; a multichannel reflected illuminator includes three fluorescent channels and a light field channel, with up to four filters installed. The trinocular head allows you to connect a digital camera for documenting and analyzing images, and the overall EX30 platform is expanded by phase contrast, dark field and simple polarization if necessary.
Optical system - infinite with correction of chromatic aberrations. PL10×/20 or PL10×/22 oculars; semi-apochromatic fluorescent lenses 4×, 10×, 20×, 40× and 100×, including options without cover glass. Fluorescent head is inclined 30°, rotates 360°, interpupular distance 50–75 mm, fixed 5:5 light split. Reflected lighting: single-color LED modules with integrated filters or 100-W mercury lamp; multi-channel system can accommodate up to four filters. The brochure shows B1/B2 modules for EGFP/GFP/FITC/Alexa Fluor 488, G1 for Texas Red/Cy3.5/MitoTracker Red, B4 for Auramine O/wtGFP and UV1/UV2 for DAPI/Hoechst/AMCA. Transmitted light uses a 3 W LED or a 6 V/30 W halogen lamp; table 150×140 or 150×162 mm, stroke 76×50 mm; microfocus accuracy 0.002 mm.
fluorescence channel (max.)
max. lens enlargement
range
mercury lamp (option)
RIF, FISH and other applications
LED modules and specification
| Optical system | infinity with correction of chromatic aberrations |
|---|---|
| Oculators | PL10×/20 mm or PL10×/22 mm |
| Objectives | semi-apochromatic fluorescent 4×/10×/20×/40×/100× (with and without cover glass) |
| Observation head. | trinocular 30°, turning 360°, interpupil distance 50–75 mm, light separation 5:5 |
| Source | monochrome LED modules with built-in filters or mercury lamp 100 W |
|---|---|
| Number of channels | Up to 4 filters at a time (multi-channel lighting) |
| LED modules | UV1/UV2 (~365 nm, DAPI/Hoechst/AMCA), B1 (~470 nm, GFP/FITC/Alexa Fluor 488), B4 (~455 nm, Auramine O/wtGFP), G1 (~560 nm, Texas Red/Cy3.5/MitoTracker Red) |
| Passing light | 3W LED or 6V/30W halogen lamp |
|---|---|
| The subject table | 150×140 mm or 150×162 mm, stroke 76×50 mm, accuracy 0.1 mm |
| Focusing | Microfocus accuracy of 0.002 mm |
For DAPI, Hoechst 33342, AMCA
For GFP/FITC/Alexa Fluor 488
For Texas Red/Cy3.5/MitoTracker Red
Special module for the detection of tuberculosis (Auramine O/wtGFP)
Up to four filters simultaneously for multiplex fluorescence
Autonomous operation over 8 hours with charge indicator
Image registration via USB 2.0