800+
target-RNA
Micro Solutions / Life Science
Direct digital counting of molecules nCounter Pro
Multiplex analysis of RNA and proteins without amplification: up to 800 targets in a single reaction
Product overview
- nCounter Pro Analysis System
- NanoString (Bruker Spatial Biology)
- USA
- nCounter
- nCounter Pro
Multiplex analysis of RNA and proteins without amplification: up to 800 targets in a single reaction
automated training station and digital analyzer based on CCD-camera
NanoString nCounter Pro from Bruker Spatial Biology is an automated direct digital counting system for reproducible multiplex analysis of RNA and proteins without enzymatic amplification. The platform is designed for translational research, biomarker development and multicenter projects.
The system consists of a training station and a digital analyzer. After hybridization, Prep Station automatically cleans the complexes and fixes them in the cartridge, and Digital Analyzer registers fluorescent molecular barcodes using microscopic optics and a CCD camera. Most panels require about 15 minutes of manual work. FFPE, freshly frozen samples, blood, plasma, serum and cell lysates are supported, and the analysis is performed in nCounter Data Analysis Pipeline or compatible cloud tools.
nCounter Pro measures 800+ RNA targets or 500+ protein targets. Recommended input is 100 ng RNA, 300 ng DNA or one protein slice; RNA control sensitivity is 0.5 fM, linearity and correlation of control additives R2 > 0.95. Prep Station processes 12 samples in about 2.5 hours, Digital Analyzer - 12 samples in 2.7 hours; Basic performance of 12-576 samples per day depending on the number of stations and the use of PlexSet. Dimensions Prep Station - 67 × 89 × 63 cm, weight 120 kg; Digital Analyzer - 66 × 66 × 48 cm, weight 67 kg. System validated to support the environment 21 CFR Part 11.
Key figures
500+
protein-target
0.5 fm
sensitivity
21 CFR Part 11
support
Applications
- Profiling of gene expression
- Search and validation of biomarkers
- Multiplex analysis of proteins
- Translational studies
- Multicentre projects
Models and configurations
- nCounter Pro
Product details
Prep Station and Digital Analyzer
fully automated
nCounter workflow
Hybridization → Cleanup → Digital Counting
Throughput
- Prep Station can handle up to 12 samples in 2.5 hours
- Digital Analyzer Reads 12 Samples in 2.7 Hours
- Up to 6 cartridges (72 samples) without operator involvement
- Multiplexing up to 96 samples in a single PlexSet reagent cartridge
Sensitivity and accuracy
- Direct digital count of molecules without enzymatic amplification
- Limit of detection of control - 0.5 fM
- Linearity and correlation of control additives R2 > 0.95
Ease of operation
- About 15 minutes of handmade work on most panels
- Support for FFPE, freshly frozen tissues, blood, plasma, serum and cell lysates
- Touch screens with step-by-step instructions at each stage
- Compatibility with environment 21 CFR Part 11
Throughput
| Multiplexing level | 800+ RNA targets or 500+ protein targets |
|---|---|
| Capacity of Prep Station | 12 samples in 2.5 hours |
| Digital Analyzer capacity | 12 samples in 2.7 hours |
| Simultaneous loading | up to 6 cartridges (72 samples) |
Sample.
| Recommended amount of material | RNA 1–100 ng, DNA 5–300 ng |
|---|---|
| Reaction volume | up to 30 μl |
| Limit of detection | 0.5 fM (15 zeptomol in 15 μl) |
| Linearity | R² > 0,95 |
Prep Station
| Dimensions (G×SH×V) | 67 × 89 × 63 cm |
|---|---|
| Weight | 120 kg |
| Power supply | 100–240 V, 50–60 Hz, 610 VA |
Digital Analyzer
| Dimensions (G×SH×V) | 66 × 66 × 47 cm |
|---|---|
| Weight | 67 kg |
| Power supply | 100–240 V, 50–60 Hz, 610 VA |
Reagents and kits
CodeSet
A ready or individual set of barcoded probes with system controls
Master Kit
Consumables and reagents for Prep Station, ready to load without additional preparation
Panel Standards
Synthetic DNA oligonucleotides to correct technical variability between studies
Low Input Kit
Profiling of up to 800 genes from a sample of 1 ng
Related equipment types
- nCounter panels and reagents
- Systems for nucleic acid isolation
- Bioinformatics analysis software
Related products
- Applied Spectral Imaging HiPath Pro — Systems for nucleic acid isolation
- BGI ZTRON — Bioinformatics analysis software
Classification
- Sequencing and genotyping
- Expression profiling and spatial biology
- Equipment for molecular biology and genetics
- Genotyping and Multiplex Platforms
- Multiplex computational expression analysis platforms
- Direct digital counting of molecules nCounter Pro