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clevergel
Micro Solutions / Life Science
Consumables for electrophoresis Cleaver Scientific
Agarose, DNA and protein markers, combs and tips for gel electrophoresis and blotting
Product overview
- Electrical consumption
- Cleaver Scientific
- Great Britain
Agarose, DNA and protein markers, combs and tips for gel electrophoresis and blotting
a set of consumables of the CleverGEL line for the daily work of the laboratory
Cleaver Scientific offers a set of consumables for gel electrophoresis and blotting: several types of CleverGEL agarose, ready-made DNA and protein markers, color-coded combs, sample tips and self-adhesive IdentiTapes labeling tapes.
The line of supplies covers the basic daily needs of the molecular laboratory. CleverGEL agarose comes in several modifications - from universal Low EEO to high-resolution PCR-grade, light melt for nucleic acid isolation and tablet for quick preparation of the gel. Ready-to-use DNA markers. The four-band and Pink Plus and Blue Wide Range protein markers contain an indicator dye and do not require additional preparation before application. The combs come in four color-coded thicknesses for preparative and high-resolution tasks, and the tips for applying samples in three versions facilitate the loading of thin PAGEs and the combing of the comb. IdentiTapes self-adhesive tapes are used to mark test tubes and samples, including storage at negative temperatures.
CleverGEL Low EEO agarose: melting point 88±1.5 °C, gelation point 36±1.5 °C, gel strength >1200 g/cm² (1% gel). High Resolution: melting ≤70 °C, strength ≥750 g/cm² (1.5% gel), fragment resolution 20–800 bp. Low Melting Point: melting ≤65 °C, gelation 26–30 °C, for fragments from 200 bp to 25 kb. DNA markers CSL-MDNA-50BP/100BP/100BPH/1KBPLUS cover 50–1500, 100–1500, 100–3000 bp and 100 bp–10 kb; load 5 µL/well, storage 6 months at 25 °C or 12 months at 4 °C. Protein markers CSL-PPL (10–175 kDa) and CSL-BBL (10–245 kDa) are loaded at 2.5–5 µL/well. Combs have thicknesses 0.75/1.0/1.5/2.0 mm, color coded black/white/red/blue. Sample-loading tips use a 33 mm microcapillary for 5 µL and tip diameters 0.57, 0.37 and 0.17 mm. IdentiTapes withstand −20…+121 °C and can be marked with a ballpoint pen, pencil or marker.
Key figures
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DNA-marker range
10-245 kDa
protein-marker range
0.75-2 mm
comb-thickness
Applications
- Electrophoresis of nucleic acids in agarose gel
- Electrophoresis of proteins (SDS-PAGE)
- Determination of molecular weight of DNA fragments and proteins
- Western-, Southern- and Northern-blotting
- Marking of samples and tubes
Models and configurations
- CleverGEL agarosa
- DNA markers
- Protein markers
- Crests.
- Sampling tips
- IdentiTapes
CleverGEL agarosa
- Standard Low EEO – for routine electrophoresis and DNA/RNA blotting 0.1–10 tb.
- High Resolution PCR grade – for analysis of fragments of 20-800 p.
- Low Melting Point - for the isolation of nucleic acids from the gel, fragments of 200 nb - 25 tb.
- Instant Agarose Tablets – Quick preparation of gel with stable concentration
DNA and protein markers
- Ready-to-use DNA markers of four ranges: 50–1500, 100–1500, 100–3,000 p.b. and 100 p.b. – 10 t.b.
- Pink Plus (10–175 kDa) and Blue Wide Range (10–245 kDa) for SDS-PAGE
Crests and tips
- Four-thick combs with color markings: 0.75 (black), 1.0 (white), 1.5 (red) and 2.0 mm (blue)
- Gell sample tips – three versions of microcapillaries for PAGE and sequencing gels
- IdentiTapes - self-adhesive marking tapes, resistant to temperatures from -20 to +121 °C
CleverGEL agarosa
| Model range | Low EEO, High Resolution (PCR grade), Low Melting Point, Instant Agarose Tablets |
|---|---|
| Melting point | 88±1,5 °C (Low EEO/Instant); ≤65 °C (LMP); ≤70 °C (High Resolution) |
| Geliac temperature | 36±1,5 °C (Low EEO/Instant); 26–30 °C (LMP); ≤33 °C (High Resolution) |
| Gel strength | >1200 g/cm2 (Low EEO/Instant, 1%); >200 g/cm2 (LMP, 1%); >750 g/cm2 (High Resolution, 1.5%) |
| Packing | 5g and 100g (Low EEO); 5g and 50g (LMP) |
DNA markers
| Ranges | 50–1500 p.N.; 100–1500 p.N.; 100–3000 p.N.; 100 p.N.—10 t.B. |
|---|---|
| Number of lanes | 17; 11; 12; 13, respectively; |
| Storage | 6 months at 25 °C; 12 months at 4 °C; 24 months at −20 °C |
| Volume of application | 5 μl/well |
| Tracking dye | Orange G, Xylene Cyanol FF, bromphenol blue |
Protein markers
| Pink Plus | 10–175 kDa, 11 bands, 10, 40 and 90 kDa reference bands |
|---|---|
| Blue Wide Range | 10–245 kDa, 12 lanes, 25 and 75 kDa reference strips |
| Volume of application | 2.5–5 μl/well |
| Storage | 3 months at 4 °C; 24 months at -20 °C |
Crests.
| Thickness (color) | 0.75 mm - black; 1.0 mm - white (standard); 1.5 mm - red; 2.0 mm - blue |
|---|---|
| Purpose | Black/white for clear high resolution bands; red/blue for increased sample volume |
Sampling tips
| Enforcement | round 0.57 mm (gels 0.75–1.5 mm); flat 0.37 mm (sequencing gels 0.4 mm); ultra-thin flat 0.17 mm (wedge gels 0.2 mm) |
|---|---|
| Microcapillary | length 33 mm, volume 5 μl |
| Compatibility | single-channel pipette |
IdentiTapes
| Material | paper-ribbon, water-, oil- and acid-resistant |
|---|---|
| Temperature range | -20 °C to +121 °C |
| Inscription | ballpoint pen, pencil, marker |
Related equipment types
- Electrophoresis (agarose) systems
- Gell documentation systems
- Western blotting kits
Related products
- Thistle Scientific omniDOC — Gell documentation systems
- Thistle Scientific chemiPRO — Western blotting kits
Classification
- Consumables
- Electrical consumption
- Equipment for molecular biology and genetics
- Electrophoresis and documentation
- Cameras and power supplies for electrophoresis
- Consumables for electrophoresis Cleaver Scientific