Micro Solutions / Life Science

Hypoxic workstation H135 HEPA / GMP insulator

Hypoxia under HEPA filtering in a 900 liter chamber, including a validated GMP execution of Grade A

Product overview

H135 HEPA / GMP
Don Whitley Scientific
Great Britain
H-series
H135 HEPA / GMP

Hypoxia under HEPA filtering in a 900 liter chamber, including a validated GMP execution of Grade A

floor model with built-in HEPA filtering; available as a validated GMP insulator

H135 HEPA/GMP is a 900-liter hypoxic workstation for cellular research in physiologically relevant atmospheres.

Hypoxystation supports controlled O2, CO2, humidity and temperature, allowing cooking, incubating and examining cultures without returning to room air. Sensory control, O2 profiles, journaling, remote access and airlock 16 liters, cycle 60 c suitable for oncology, neuro- and cardiac studies. HEPA model exceeds ISO 14644 Class 3 requirements for the clean atmosphere inside the chamber. The designation in the source file combines HEPA and GMP: the GMP execution is a validated insulator with a Grade A/ISO 3 unidirectional stream.

chamber volume 900 liters; CO2 input, air and N2; airlock 16 liters, cycle 60 s. The family controls O2 up to 20% and CO2 up to 15% in increments of 0.1% in the respective configurations. Dimensions Sh×G×B 1473×1056×1063 mm, weight 175 kg; options HEPA, humidification, cooling and vacuum selection depend on execution.

Key figures

900 l.

chamber volume

HEPA

Included in ISO 14644 Class 3

ISO 3

GMP execution, unidirectional stream Grade A

16 l.

lock, 60s cycle

Applications

Oncological research
Working with stem cells
ATMP and Cell Therapy (GMP-performance)
Cell and tissue culture
Visualization of living cells inside the chamber

Models and configurations

H135 HEPA / GMP

Product details

H135 HEPA workstation

highest and deepest camera line

Removable front panel

2 or 3 ports of access

HEPA performance (Hypoxystation)

The highest, widest and deepest hypoxic chamber of the line, useful volume of about 900 liters
It accommodates equipment for working inside the camera: visualization systems of living cells, microscopes, microplate readers
Large removable front panel with two or three ports
Whitley's built-in HEPA filtering system as standard
Advanced Biological Containment (EBD)
Two optional cooling options: remove heat from the equipment in the chamber or cool the atmosphere below 10 °C
16-liter nitrogen-blown lock to eliminate the risk of breach of chamber conditions
Connection to the Whitley i2 Instrument Workstation

GMP performance (Grade A insulator)

Precise control of O2 (0.1–20.0%) and CO2 (0.1–15.0%) for effective cultivation conditions
HEPA H14 filter and unidirectional airflow support EU GMP Grade A
Recycling technology creates ISO 3 environment – 100 times fewer particles than Grade A/ISO 5
Continuous monitoring of viable and non-viable particles with microbiological air sampling
Built-in VHP biodecontamination with isolated camera processing in adjacent modules
Audit recording of all user inputs, events and alarms for data traceability

HEPA performance – camera and airlock

Chamber volume	900 l.
Lock capacity	16 l.
Lock cycle	60.
Dimensions (W×D×H)	1473×1056×1063 mm
Weight	175 kg

Atmospheric control (both performances)

Gas supply	CO2/air/N2
Range O2	0.1-20.0%, step 0.1%
CO2 range	0.1–15.0%, step 0.1%
Atmospheric purity class	exceeds ISO 14644 Class 3 (HEPA); ISO 3 in GMP

GMP insulator - camera and airlock

Chamber volume	900 l.
Lock capacity	17.5 l or 43 l
Lock cycle	1-5 minutes
Port system	2 hand ports
Dimensions (W×D×H)	1500×1130×1080 mm
Weight	295 kg

Options for HEPA-execution

Enhanced Biological Containment

Enhanced biological protection for working with pathogenic samples

Cell cooling system

Removal of equipment heat or cooling of chamber atmosphere below 10 °C

Whitley Transfer Tunnel

Connection to Whitley i2 Instrument Workstation without air contact

Options of GMP insulator

Upgrade ULPA filter

Higher air filtration class instead of HEPA H14

Three-way airlock (3-way airlock)

Additional route for transferring materials between modules

Cooling module (refrigeration)

Storage of reagents for cell culture in Grade A conditions

Quick-removal glove system

Replace gloves without loss of conditions and risk of contamination

Related equipment types

Class A2 Biosafety Boxes
CO2 incubators
Living cell imaging systems
Equipment for GMP-production of cell drugs

Classification

Cellular technology and ART
Hypoxia equipment
Microbiological equipment
Cultivation
Hypoxic workstations
H135 HEPA / GMP