Cytek
Spectral flow cytometer series Cytek Northern Lights
Scalable spectral cytometry: from a single laser to 24-color panels
A scalable spectral cytometer for everyday cellular research, from basic tests on a single laser to high-parameter immunoprofiling. Northern Lights brings Full Spectrum Profiling into a compact and cost-effective configuration.
Request a quotationThe system is suitable for proliferation, apoptosis, cell cycle, viability assessment and multicolor immunoprofiling. The full spectrum of the signal allows the use of fluorochromes with close emission maxima, extract autofluorescence and transfer panels between instruments of the Cytek ecosystem; SpectroFlo combines QC, collection and spectral separation.
Configurations with 1, 2 or 3 lasers are available. Single laser supports panels of up to 9 colors, dual laser contains 30 fluorescent detectors and demonstrates 21-color panels, three laser - up to 24 colors. The configuration is selected for the required complexity of the panel and can include an automatic loader of tubes and microplates.
Flexibility of configuration
- Configurations with 1, 2 or 3 lasers are available, from basic tests to high-parameter immunoprofiling.
- Single-laser system supports panels up to 9 colors, dual-laser (V/B) – up to 21 colors, three-laser – up to 24 colors
- Panels are transferred between Cytek ecosystem appliances without re-optimization
Analysis opportunities
- The full emission spectrum of each fluorochrome allows the use of dyes with close emission maxima.
- Autofluorescence Extraction Increases Sensitivity to Low-Expression Markers
- SpectroFlo combines quality control, data collection and spectral signal separation into a single workflow.
Throughput
- Data logging speed up to 35,000 events/s (on a 3-laser system)
- ASL Auto Booter Processes 96-well Microplate in 27 Minutes, AMS in 35 Minutes
- Violet SSC detector (in 3-laser configuration) supports particle resolution from 0.2 μm
Lasers and detectors
| Parameter | 1 laser | 2 lasers (B/R) | 2 lasers (V/B) | 3 lasers |
|---|---|---|---|---|
| Lasers | 488 nm (50 mW) | 488 nm (50 mW) + 640 nm (80 mW) | 405 nm (100 mW) + 488 nm (50 mW) | 405 nm (100 mW) + 488 nm (50 mW) + 640 nm (80 mW) |
| Fluorescent channels | 14 (Blue) | 22 (Blue+Red) | 30 (Violet+Blue) | 38 (Violet+Blue+Red) |
| Demonstrated panels | 9-colour | — | 21 colors | 24-colour |
Throughput
| Parameter | 1 laser | 2 lasers (B/R) | 2 lasers (V/B) | 3 lasers |
|---|---|---|---|---|
| Speed of registration | Up to 35,000 events/s (3-laser system) | |||
| Linearity | FITC R²≥0,995 / PE R²≥0,995 | |||
| Side scattering authorization | particles from 0.2 μm (violet SSC - only if there is a 405 nm laser) | |||
| Leapthrough sample (carryover) | <0.1% manually with tubes; on microplate: ≤0.3% standard / ≤0.1% low-carryover / ≤1% high-throughput |
Construction and connection
| Parameter | 1 laser | 2 lasers (B/R) | 2 lasers (V/B) | 3 lasers |
|---|---|---|---|---|
| Sample format | 12×75 mm tubes; ASL - 96, 96 deep-well microplates and 40-tube tripod; AMS - microplate 96 | |||
| Speed of loader | ASL – 27 mins per 96-well microplate; AMS – 35 mins | |||
| Dimensions | without loader 54×52×52 cm; with loader 58×62×52 cm | |||
| Weight | 61 kg (3-laser configuration) without loader; loader – 13 kg | |||
| Power supply | 100–140 V, 15 A or 200–250 V, 10 A; heat generation 500 W |