64
fluorescent detector
Micro Solutions / Life Science
Spectral flow cytometer Cytek Aurora Evo
New generation Aurora: automation, feed speeds up to 200 μl/min and instrument harmonization
Product overview
- Cytek Aurora Evo
- Cytek
- USA
- Aurora
- Cytek Aurora Evo
New generation Aurora: automation, feed speeds up to 200 μl/min and instrument harmonization
Full Spectrum Profiling for High-Term Laboratories
Cytek Aurora Evo is a next-generation spectral flow cytometer for high-parameter cell analysis, automated research and high-load laboratories. The platform develops Full Spectrum Profiling and preserves the portability of Aurora ecosystem panels.
The system records the full spectrum of each fluorochrome and performs spectral separation of signals, allowing you to build complex multicolor panels without constantly reconfiguring optical filters. Aurora Evo supports test tubes and microplates, accelerated sample delivery, standardized configuration and harmonization of instruments. The extended light scattering range allows for the analysis of both small particles and large cells in a single protocol.
The official Aurora Evo card is configured with up to 5 lasers and contains up to 64 fluorescent detectors. The maximum reported sample rate is up to 200 μl/min when working with test tubes and microplates. The platform uses Full Spectrum Profiling technology, automated quality control and SpectroFlo for control. The specific configuration of lasers, channels, loader and automation is selected according to the official technical specification of the manufacturer.
Key figures
65 000
events/s (max.)
24 minutes
96-hole plate
200
μl/min, max. feed rate
Applications
- High-parametric cell analysis
- Automated and Multicenter Research
- High-load laboratories
- Harmonization of devices between sites
Models and configurations
- 2 lasers
- 3 lasers
- 4 lasers
- 5 lasers
Product details
Compact platform with built-in loader
64.5 × 61.0 × 60.0 cm
Automation and productivity
- Sample feed rate up to 200 μl/min for test tubes and microplates while maintaining resolution
- Automatic start and stop of the device simplify the daily work of the laboratory
- Built-in flowmeter provides an absolute count of cells without counting granules
Data reproducibility
- Standardized equipment tuning and harmonization provide comparable data across instruments, sites and time.
- Extended dynamic lateral scattering range allows small particles and large cells to be analyzed in a single protocol
- Low-noise electronics improve the resolution of weak signals
Software
- Separate regimes for conventional compensation and spectral unmixing
- Autofluorescence Explorer - a managed workflow for autofluorescence extraction
- 21 CFR Part 11 Compliance Tools; Cytek Cloud with SpectroPanel, Panel Builder and Experiment Builder for remote panel design
Lasers and detectors
| Lasers | 488 nm (50 mW) + 640 nm (80 mW) 405 nm (100 mW) + 488 nm (50 mW) + 640 nm (80 mW) 405 nm (100 mW) + 488 nm (50 mW) + 561 nm (50 mW) + 640 nm (80 mW) 355 nm (20 mW) + 405 nm (100 mW) + 488 nm (50 mW) + 561 nm (50 mW) + 640 nm (80 mW) |
|---|---|
| Fluorescent channels | 22 (Blue+Red) 38 (Violet+Blue+Red) 48 (+ Yellow-Green) 64 (+ UV) |
| Detection range | 498–829 nm 420–829 nm 420–829 nm 365–829 nm |
Throughput
| Nominal speed of registration | up to 35,000 events/s |
|---|---|
| Maximum speed of registration | up to 65,000 events/s |
| Sensitivity (MESF) | FITC ≤5, PE ≤4, APC ≤3, Pacific Blue ≤4 |
| Linearity | FITC R²≥0,995 / PE R²≥0,995 |
| Sample rate | 15/30/60/100/200 μl/min (Low/Medium/High/Ultra/Max) |
| Pipeline-sampling | standard mode ≤0.3%; low speed mode ≤0.1% |
Construction and connection
| Sample format | 12×75 mm tubes; 96, 96 deep-well, 384; 40-tube tripod |
|---|---|
| Speed of loader | 24 min on 96-well microplate in high-performance mode |
| Dimensions | 64.5 × 61.0 × 60.0 cm |
| Weight | 95 kg (5-laser configuration) |
| Power supply | 100–140 V, 15 A or 200–250 V, 10 A; heat generation 500 W |
| Computer | Intel Core i5-13500 or analogue, 64 GB of RAM, SSD 1 TB + 2 TB, monitor 32" 4K UHD |
Cytek Cloud Tools
Full Spectrum Viewer
Selection of fluorochromes for device configuration
Panel Builder / SpectroPanel
Automated and manual design of multicolor panels
Experiment Builder
Creating SpectroFlo experiments remotely, outside the instrument
Related equipment types
- Biosafety cabinets
- CO2 incubators
- Centrifuges for cell cultures
- Automatic cell counters
Related products
- Heal Force HFsafe 900 — Biosafety cabinets
- BioAir SafeGrow PRO — CO2 incubators
- Cence TG16-WS — Centrifuges for cell cultures
- IUL SphereFlash® — Automatic cell counters
Classification
- Immunology
- Spectral flow cytometers
- Equipment of cell technologies and reproductive medicine
- Analysis of cellular suspension
- Flow cytometers, including spectral
- Cytek Aurora Evo