Micro Solutions / Life Science

Visualizing flow cytometer Amnis ImageStreamX Mk II

Microscopic image of each cell with statistical flow cytometry power

Product overview

Amnis ImageStreamX Mk II
Cytek (Amnis)
USA
Amnis ImageStream
Amnis ImageStreamX Mk II

Microscopic image of each cell with statistical flow cytometry power

Up to 12 channels of image and 10 fluorescence colors per cell

A visualizing flow cytometer that combines the statistical power of flow cytometry with a microscopic image of each cell. Amnis ImageStreamX Mk II is suitable for complex studies of morphology, spatial signal localization, and rare cellular events.

The system quickly collects millions of images of cells, registers bright field, lateral scattering and fluorescent channels, performs pixel spectral compensation, and links quantitative graphs to cell galleries. IDEAS and Amnis AI support colocalization, nuclear translocation, internalization, spot counting, shape analysis, and intercellular analysis. Interactions, as well as training classifiers based on CNN, Random Forest and transfer learning.

Up to 12 cellular imaging channels and up to 10 fluorescence colors; 20x, 40x and 60x lenses. 6 or 12 channel configurations available, up to 6 lasers, High Gain mode, Extended Depth of Field and autosampler for 96-well microplates. 20-200 μl sample size, sample efficiency up to 95%; dimensions 889 x 660 x 635 mm, weight 182 kg.

Key figures

12

picture

60x

maximum

6

laser-maximum

95%

sample-use

Applications

Morphology and spatial signal localization
Colocalization and nuclear translocation
Internalization and spot counting
Rare cellular events
Classifying images with AI

Models and configurations

6-channel configuration
12-channel configuration

Product details

Scope of application

morphology, signal localization, rare cellular events

Image and analysis

The system records bright-field image, lateral scattering and fluorescent channels for each cell, performing pixel spectral compensation.
IDEAS and Amnis AI support colocalization, nuclear translocation, internalization, stain counting, shape analysis and intercellular interactions
Classifier training available on CNN, Random Forest and transfer learning

Instrument configuration

6 or 12 channel configurations (second camera upgrade)
Up to 6 lasers, High Gain and Extended Depth of Field mode to expand range by focus
MultiMag option adds 20x and 60x lenses to standard 40x with autofocus when changing the magnification

Capacity

Rapidly Collecting Millions of Cell Images in a Single Sample
Autosampler for 96-well microplates
Sample volume of 20–200 μl with utilization efficiency up to 95%

Optics and imagery

Image channels	6 12
Fluorescence colors	— 10
Objectives	20x, 40x, 60x (with MultiMag option); High Gain mode
Lasers	up to 6 lasers

Throughput

Sample size	20–200 μl
Efficiency of sample use	95%
Autosampler	96-hole microplate

Design

Dimensions	889 × 660 × 635 mm
Weight	182 kg

Configuration options

MultiMag Option (20x/60x lenses)

Expands the range of magnification relative to the standard 40x with autofocus when changing

Autosampler for 96-well microplates

Automatic sampling from the microplate

High Gain/Extended Depth of Field Mode

Increases sensitivity and depth of field when taking cell images

Related equipment types

Fluorescent microscopes
Biosafety cabinets
Centrifuges for cell cultures
Automatic cell counters

Classification

Immunology
Visualizing flow cytometry
Equipment of cell technologies and reproductive medicine
Analysis of cellular suspension
Flow cytometers, including spectral
Amnis ImageStreamX Mk II