Micro Solutions / Life Science

Visualizing flow cytometer Amnis FlowSight

Compact imaging cytometry: up to 4,000 objects per second with each cell image

Product overview

Amnis FlowSight
Cytek (Amnis)
USA
Amnis FlowSight / CellStream
Amnis FlowSight

Compact imaging cytometry: up to 4,000 objects per second with each cell image

12 detection channels, 1 to 4 lasers

A compact imaging flow cytometer for laboratories that need quantitative cytometry to image each cell. Amnis FlowSight combines the statistical power of flow cytometry with a microscopic image, allowing immediate confirmation of population morphology and spatial distribution of signals.

The device records fluorescence, bright field and lateral scattering in 12 channels and links each graph point or gate to the corresponding image of the cell. IDEAS software provides ready-made workflows for apoptosis, cell cycle, colocalization, internalization, nuclear localization, shape change and stain counting. Machine learning tools, Amnis AI and software versions for regulated environments are available.

Visualization speed up to 4,000 objects/s; 20x lens, numerical aperture 0.6, pixel size 1.0 × 1.0 μm, field of view 60 × 256 μm Sample volume 20–200 μl, sample efficiency up to 95% Standard laser 488 nm, optionally available 405, 561 and 642 nm – up to a maximum of 4 lasers in configuration; Standard lateral scattering channel – 785 nm Dimensions 457 × 465 × 635 mm, weight 61 kg, consumption 450 W (100-240 V, 50/60 Hz); external air and water connections are not required.

Key figures

12

detection channels

4 000

facility

61 kg

mass

4

laser-maximum

Applications

Quantitative cytometry with cell image
Apoptosis and the cell cycle
Colocalization and nuclear translocation
Counting spots and changing the shape of the cell

Models and configurations

Amnis FlowSight

Product details

FlowSight configurations

variants of lasers and instrument options

Image and analysis

Fluorescence, bright field and lateral scattering in 12 channels with each graph point tied to the cell image
IDEAS software includes ready-made workflows for apoptosis, cell cycle, colocalization, internalization, nuclear localization, shape changes and spot counting.
Machine learning, Amnis AI and software versions for regulated environments are available

Compact platform

Standard 488 nm laser, optionally 405, 561 and 642 nm (up to 4 lasers in configuration)
20x lens with a numerical aperture of 0.6, field of view 60 × 256 μm
No need for external air or water connection

Optics and imagery

Channels	12
Objective	20x, numerical aperture 0.6
Pixel size	1.0 × 1.0 μm
Field of vision	60 x 256 μm
Speed of visualization	up to 4,000 objects/s
Lasers	488 nm standard; optionally 405, 561 and 642 nm (up to 4 lasers)
Side scattering (SSC)	785 nm - standard

Test and construction

Sample size	20–200 μl
Efficiency of sample use	95%
Dimensions	457 x 465 x 635 mm
Weight	61 kg
Consumption	450 W, 100–240 V, 50/60 Hz
Premises requirements	No external air and water are required

Consumables and kits

FlowSight Calibration Reagent

Calibration reagent for setting the device before data collection

Amnis NF-κB Translocation Kit

Reagent kit and protocol for analysis of NF-κB translocation into the cell nucleus

Related equipment types

Fluorescent microscopes
Biosafety cabinets
Centrifuges for cell cultures
Automatic cell counters

Classification

Immunology
Visualizing flow cytometry
Equipment of cell technologies and reproductive medicine
Analysis of cellular suspension
Flow cytometers, including spectral
Amnis FlowSight