4
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Micro Solutions / Life Science
Chroma 69014 ET DAPI/Aqua/Green/Orange FISH
Four-band set for simultaneous visual viewing of DAPI, Aqua, Green and Orange
Product overview
- 69014 ET DAPI/Aqua/Green/Orange FISH
- Chroma
- USA
- ET Multi-Band FISH
- 69014
Four-band set for simultaneous visual viewing of DAPI, Aqua, Green and Orange
ET Multi-Band series, designed for visual viewing, not for camera registration
Chroma 69014 extends the simultaneous review of the FISH drug to DAPI, Aqua, Green and Orange. The weakened excitation of DAPI helps maintain the visibility of the Aqua signal, which is otherwise easily lost amid bright nuclear contrast.
The kit is designed to simultaneously visually observe DAPI and three color FISH channels Aqua, Green and Orange. The four-band excitation filter uses a reduced level of excitation DAPI to prevent DAPI contrasting from overriding the Aqua signal, with DAPI and Aqua using one emission band. The configuration is designed to be viewed simultaneously, but not to work with the camera.
The set composition: 69014x - four-band excitation filter (EX), 69011bs - multiband dichroic beam splitter (BS), 69011m - multiband emission filter (EM). Spectral configuration covers DAPI / Aqua / Green / Orange; DAPI and Aqua share one emission band, so the set is not designed to separate camera recording of the two The components are from the high-performance Chroma ET line with typical 93-97% transmission, steep fronts and subpixel alignment, the light source type and filter cube format are agreed separately.
Key figures
93–97%
ET-pass
Applications
- Cytogenetics
- FISH diagnostics
- Multicolor visual viewing of the drug
- Fluorescence microscopy
Models and configurations
- 69014
Product details
Set card 69014
catalogue
Transmission spectrum
four-window
Optical circuit
- Four-band excitation filter 69014x
- Multi-band dichroic light splitter 69011bs
- Multi-band emission filter 69011m
Features
- Reduced DAPI excitation retains visibility of weaker Aqua signal
- DAPI and Aqua share common emission band
- The configuration is designed for simultaneous visual viewing, not for camera registration.
Optical parameters
| Spectral windows | DAPI, Aqua, Green, Orange |
|---|---|
| Passage | typically 93-97% |
| Limitation | DAPI and Aqua share a common emission band – a set not for separate camera recording of these signals. |
Composition of the set
| Excitation filter (EX) | 69014x |
|---|---|
| Light splitter (BS) | 69011bs |
| Emission filter (EM) | 69011m |
| Series | 69000 ET Multi-Band |
Related equipment types
- Fluorescent microscopes
- Light filter kits for FISH
- Light sources for fluorescence microscopy
Related products
- Ningbo Sunny Instruments EX30 — Fluorescent microscopes
- Chroma 49000 ET DAPI — Light filter kits for FISH
- Thistle Scientific powerPRO — Light sources for fluorescence microscopy
Classification
- Cytogenetics
- Light filter kits for FISH
- Microscopy and imaging systems
- Optical components
- Filter sets and dichroic mirrors for fluorescence
- 69014 ET DAPI/Aqua/Green/Orange FISH