93–97%
ET-pass
Micro Solutions / Life Science
Chroma 49000 ET DAPI light filter set
Single-channel set of filters for bright visualization of DAPI-contrast cores
Product overview
- 49000 ET DAPI
- Chroma
- USA
- ET Single-Band
- 49000
Single-channel set of filters for bright visualization of DAPI-contrast cores
ET Single-Band series for cytogenetics and control of FISH drugs
Chroma 49000 ET DAPI is a single-channel set of light filters for bright imaging of DAPI in wide-field fluorescence microscopy. In a cytogenetic protocol, it is suitable for confident observation of DAPI contrasting chromosomes and cell nuclei.
The kit forms a separate optical DAPI channel: the excitatory filter emits the ultraviolet range, the dichroic divider directs the excitatory light to the sample, and the emission filter passes blue fluorescence to the eyepieces or detector. The single-channel configuration is convenient for checking the contrast quality and sequential recording. DAPI is separate from color FISH probes.
The composition of the set according to the official Chroma catalog: AT350/50x V2 - excitatory filter (EX), T400lp - dichroic beam splitter (BS), ET460/50m - emission filter (EM). Nominal excitation band - 350/50 nm, emission band - 460/50 nm; beam splitter - long-transmitting 400 nm. The model belongs to the 49000 series ET Single-Band for non-laser For ET filters, the manufacturer specifies typical 93-97% transmission, steep spectral fronts and structural subpixel alignment of the set, and compatibility with a specific microscope and filter holder must be confirmed when ordering.
Key figures
1
channel
Applications
- Cytogenetics
- FISH diagnostics
- Quality control of DAPI-contrast
- Fluorescence microscopy of nuclei
Models and configurations
- 49000
Product details
The 49,000 ET DAPI Dial Card
catalogue
Transmission spectrum
excitation/light splitter/emission
Optical circuit
- Excitatory filter AT350/50x V2 emits ultraviolet range
- Dichroic T400lp light splitter directs excitatory light to sample
- ET460/50m filter passes blue DAPI fluorescence to detector
Quality.
- Typical ET filter transmission 93–97%
- Steep spectral fronts of stripes
- Subpixel combination of components of the set
Optical parameters
| Excitation band | 350/50 nm |
|---|---|
| light-splitter | long-transmitting, 400 nm |
| Emission band | 460/50 nm |
| Passage | typically 93-97% |
Composition of the set
| Excitation filter (EX) | AT350/50x V2 |
|---|---|
| Light splitter (BS) | T400lp |
| Emission filter (EM) | ET460/50m |
| Series | 49000 ET Single-Band |
Related equipment types
- Fluorescent microscopes
- Light filter kits for FISH
- Light sources for fluorescence microscopy
Related products
- Ningbo Sunny Instruments EX30 — Fluorescent microscopes
- Chroma 59002v2 ET DAPI/Orange FISH — Light filter kits for FISH
- Thistle Scientific powerPRO — Light sources for fluorescence microscopy
Classification
- Cytogenetics
- Light filter kits for FISH
- Microscopy and imaging systems
- Optical components
- Filter sets and dichroic mirrors for fluorescence
- 49000 ET DAPI