<20 nm
lateralization
Micro Solutions / Life Science
Vutara VXL ultra-high resolution microscope
Localization of individual molecules with a resolution of better than 20 nm - in 3D and with multiplex labeling
Product overview
- Vutara VXL
- Bruker
- USA
- Vutara
- Vutara VXL
Localization of individual molecules with a resolution of better than 20 nm - in 3D and with multiplex labeling
SMLM workstation for STORM, PALM and PAINT with bi-plane detection and SRX software
Compact multimodal SMLM platform for quantitative 3D ultra-resolving imaging of cells and tissues.
Vutara VXL localizes individual molecules by STORM, PALM, PAINT and SPT methods, simultaneously registering two focal plans. SRX leads the user from calibration and shooting to 3D reconstruction and quantitative analysis; optional PlexFlo microfluidics supports serial multiplex labeling.
Declared lateral resolution better than 20 nm and axial 50 nm for bi-plane detection. Field of view is 50×50 μm for 3D SMLM and 200×200 μm for widefield or multiplex localization shooting; supported up to five consecutive colors, 60×/NA 1.3 objective and a typical imaging depth of more than 50 μm depending on the sample.
Key figures
50 nm
axial, biplane
5
lineage
>50 μm
depth
Applications
- Neuroscience and synapses
- Spatial genomics and chromatin tracing
- Virology
- Cell Biology and Protein Colocalization
- Tracking particles in living cells
- Multiplex proteomics and transcriptomics
Models and configurations
- Vutara VXL
Product details
Vutara VXL
compact workstation SMLM
Quantitative analysis in SRX
clusters, colocalization, distributions
Nanoscale resolution
- PALM, STORM/dSTORM, PAINT and DNA-PAINT
- Biplane technology: simultaneous shooting of two focal planes for 3D localization
- Top-hat lighting from square fiber gives uniform excitation for quantitative data
- Active correction of focus drift during shooting
Multiplexing
- Microfluidics PlexFlo is controlled from SRX and alternates cycles of tagging and shooting
- Replaceable vials with probes allow visualizing an almost unlimited number of targets
- OligoSTORM, RefreshSTORM, DNA-PAINT, multiplex antibody labeling
Analysis of SRX data
- Spatial distribution: Ripley K function, pair correlation, nearest neighbors
- Cluster analysis, colocalization and resolution evaluation
- Tracking in living cells: medium-square and angular displacement
Permission and field of view
| SMLM Permission | better than 20 nm by XY; 50 nm by Z (Biplane) |
|---|---|
| Multiplane survey | 2 focal planes at the same time, the difference in depth of 1 μm; more - in the Z-stack mode |
| Depth of visualization | >50 μm typical, depending on the sample |
| Field of vision | 50 × 50 μm – SMLM and 3D localization; 200 × 200 μm – multiplex localization and wide field; more – crosslinking tiles |
Optics and detection
| Excitation lasers | 405 nm 120 mW; 555 nm 2000 mW; 638 nm 1200 mW; optionally 488 nm 2000 mW and 750 nm 1500 mW |
|---|---|
| Multicolor photography | Up to 5 colors sequentially; 2 colors simultaneously with two cameras (optional) |
| Camera. | sCMOS Orca Flash 4.0 V3; optional Orca Fusion BT |
| Objective | 60×, NA 1.3, silicone immersion, working distance 0.3 mm, cover glass #1.5H |
| Regimes | SMLM, broadfield localization, epifluorescence, passing light |
Mechanics
| XY-table | stroke 100 × 50 mm, optical encoder with a resolution of 1 nm |
|---|---|
| Focus stability | drift <30 nm in 10 minutes; correction of XY drift after shooting |
POs and options
| Software | SRX: shooting, 3D localization of measured PSF, particle tracking, statistical analysis, visualization |
|---|---|
| Microfluidic PlexFlo | 15 samples + buffer, tanks 2 ml; or 96 samples + buffer |
| Incubation | Control of temperature, CO2 and humidity for living cells (optional) |
Options
Microfluidic PlexFlo
Sequential multiplex tagging and washing in shooting cycles
Incubation chamber
Controlling Temperature, CO2 and Humidity for Living Cells
Lasers 488 nm and 750 nm
Expansion of fluorophores
Orca Fusion BT Camera
Alternative sCMOS camera; second camera for simultaneous two-color shooting
Network Storage (NAS)
Storage of large localization data sets
Related equipment types
- Anti-vibration tables
- CO2 incubators
- Workstations for image analysis
- Biosafety cabinets
Related products
- Astec DHS — CO2 incubators
- Heal Force HFsafe 900 — Biosafety cabinets
Classification
- Immunology
- Ultra-high resolution microscopes
- Microscopy and imaging systems
- Fluorescent microscopes and modules
- Vutara VXL