~600 microns
field of view, 20x
Micro Solutions / Life Science
Multiphoton microscope Ultima in Vitro
Visualization, photoactivation and electrophysiology on slices: up to 16 analog channels in one system
Product overview
- Ultima In Vitro
- Bruker
- USA
- Ultima
- Ultima In Vitro
Visualization, photoactivation and electrophysiology on slices: up to 16 analog channels in one system
Multiphoton system based on Nikon, Olympus and Zeiss microscopes for slices and cultures
Multiphoton system for slices, cell cultures and combined optical and electrophysiological experiments.
Ultima In Vitro combines visualization, photoactivation and recording of electrical signals. The second scanning path, synchronization of external devices and Prairie View allow you to build complex protocols for neural networks and tissue explant.
Field of view is about 600×600 μm with a 20× lens. Raster scanning reaches 1.65 fps at 512×512, optionally 30 fps with a resonance scanner; optical input is optimized for 690–1300 nm.
Key figures
30 frames/s
512×512, resonance scanner
690-1300 nm
multiphoton
16
analogue inputs/outputs
Applications
- Electrophysiology on sections of the brain
- Ancading and photoactivation
- Neural networks in vitro
- Tissue explant
- Cell culture
Models and configurations
- Ultima In Vitro
Optics and electrophysiology together
- Built-in control and registration of electrophysiology – up to 16 analog inputs/outputs
- The second set of electroplating for ancading in parallel with the shooting
- Dodt detector for DIC-like contrast of unpainted slices
Scan speed
- Raster and spiral scanning: up to 6 fps at 512×512 in spiral mode
- 8 kHz resonance scanner: 30 fps at 512×512 and more than 1300 fps for 512×8 ROI
Flexible platform
- Installed on Nikon Ti-E, Ni-E, Olympus BX51WI/BX61WI, Zeiss Axio Observer/Examiner
- Up to 4 reflected PVCs with upgrade to GaAsP
- Piezofocus with an accuracy of 0.05 μm
Scanning head.
| Method of scanning | Cambridge 6mm pair of electroplating, raster and spiral scanning |
|---|---|
| Field of vision | ~600 × 600 μm with a 20× lens |
| Speed (raster) | 1.65 fps at 512×512 >12 fps at 64×64 |
| Speed (spiral) | 6 fps at 512×512; ~30 fps at 64×64 |
| Ankajing (option) | second set of Cambridge 3 mm galvanometers |
| Speed shooting (option) | 8 kHz resonance galvanometer; 30 fps at 512×512; >1300 fps for ROI 512×8 |
Detectors.
| The reflected channel | 1-4 Hamamatsu Multi-Alkali, upgrade to GaAsP |
|---|---|
| The passing channel | 1-2 Hamamatsu Multi-Alkali |
| Dodt / passing light | one-way Hamamatsu |
Optical inputs and platform
| Multiphoton laser | 690-1300 nm |
|---|---|
| Laser safety class | Class 1 |
| Basic microscopes | Nikon Ti-E, Ni-E; Olympus BX51WI, BX61WI; Zeiss Axio Observer/Examiner |
| Piezofocus. | range of 150 μm, accuracy of 0.05 μm |
Electrophysiology
| Analog inputs/outputs | 16 |
|---|---|
| Software | Prairie View |
Options
Arcade module
Second set of 3 mm galvanometers for photo manipulation
8kHz resonance scanner
High-speed photography
GaAsP detectors
Increased sensitivity of the reflected channel
Related equipment types
- Femtosecond lasers for multiphoton microscopy
- Electrophysiology systems (patch-camp)
- Anti-vibration tables
- Incubation chambers for microscopes
Related products
- Thistle Scientific multiSUB — Incubation chambers for microscopes
Classification
- Immunology
- Multiphoton microscopes
- Microscopy and imaging systems
- Fluorescent microscopes and modules
- Ultima In Vitro