Micro Solutions / Life Science

Multiphoton microscope Ultima Investigator Plus

Deep in vivo imaging with a wide field of 1.4 × 1.4 mm and the possibility of growth up to Ultima 2Pplus

Product overview

Ultima Investigator Plus
Bruker
USA
Ultima
Ultima Investigator Plus

Deep in vivo imaging with a wide field of 1.4 × 1.4 mm and the possibility of growth up to Ultima 2Pplus

Basic multiphoton system with wide-angle signal collection and modular options

Modular multiphoton microscope with a large field of view for deep in vivo imaging of tissues.

The system is optimized for living objects and combines wide-angle signal collection with an expandable platform, and optogenetics, high-speed scanning, FLIM, multi-area photography and subsequent upgrade to Ultima 2Pplus are available.

The resonance scanner supports 30 fps at 512×512. For Z-positioning, a piezo drive is available with a step of 0.1 μm and a range of 150 μm; the motorized XY table has a stroke of 6 inches X and 3 inches Y.

Key figures

1.4 mm

field of view, 16x

30 frames/s

512×512, resonance scanner

2P / 3P

laser-entry

→ 2Pplus

field-upgradation

Applications

Neuroscience
Intravital imaging in immuno-oncology
T-cell mechanisms
Collagen and tissue structure
Calcium and Voltage Imagery
Three-photon imaging

Models and configurations

Ultima Investigator Plus

Product details

Ultima Investigator Plus

large gap for working with animals

Tissue imaging

multi-pole editing and deep shooting

Depth and sensitivity

Wide-angle signal collection increases signal-to-noise ratio in depth of scattering tissues
Detectors placed close to the sample; upgrades to GaAsP available
Low-dispersion optical input is designed for two- and three-photon excitation

Modularity

Options for optogenetics, high-speed scanning and FLIM
Multi-regional scanning for rapid transition between target areas
Upgrading to Ultima 2Pplus workstation at the installation site

Programme management

Prairie View with full control of signal speed, resolution and intensity
PrairieLink transmits data to external programs and receives script commands during shooting.
Synchronization with stimulators and recording systems for closed-loop experiments

Scanning.

Method of scanning	pair of 6 mm galvanometers
Field of vision (galvano)	16×: 1.4×1.4 mm; 40×: 495×495 μm; 60×: 160×160 μm
Field of vision (resonance)	16×: 982×982μm; 40×: 353×353μm
Raster speed (zoom 1x)	2048×2048 – 0.334 fps; 1024×1024 – 0.787 fps; 512×512 – 1.572 fps; 64×64 – 12.329 fps
Time for the pixel	0.4–100 μs in increments of 0.4 μs
Scanning setup	1–2048 pix/line and rows/frames; zoom to 128; turn 360°; linear and point scans
Resonance scanner (option)	8 kHz; 30 fps at 512×512

Detection and optics

Detectors.	2 Multi-Alkali FEUs; optionally GaAsP Hamamatsu or 4 upper non-descanned detectors
Optical entrance	ultrashort pulse IR laser (2P or 3P), laser is not included
Epifluorescence	dichroic-mirror

Motorization

Z-focus.	stroke of 25 mm, minimum pitch of 0.025 μm
Z-pieze	0.1 μm step; range 400 or 1000 μm
Motorized base (option)	XY-movement ~19 mm (0.75 inches), step 0.3 μm
Motorized XY table	≈152 × 76 mm (6 × 3 inches), step from 0.3 μm, accuracy 1 μm

Controls

Computer	specialized workstation
Software	Prairie View, PrairieLink
Imput/output	high-performance analog inputs/outputs, GPIO block, digital synchronization

Options

resonance scanner

30 fps at 512×512, ROI with panning

GaAsP detectors

Hypersensitivity

TCSPC FLIM

Visualization of the life time of fluorescence

LED lighting

Full photoactivation

Pockels' cell

Software power management of laser

OptoVolt Module

Voltage-image

Motorized revolver head

Off-axis tissue imaging

Inverter Module

Translation to an inverted configuration

Related equipment types

Femtosecond lasers for multiphoton microscopy
Electrophysiology systems (patch-camp)
Anti-vibration tables
Incubation chambers for microscopes

Classification

Immunology
Multiphoton microscopes
Microscopy and imaging systems
Fluorescent microscopes and modules
Ultima Investigator Plus