FN 28 mm
field
Micro Solutions / Life Science
Multiphoton microscope Ultima 2Pplus
FN 28mm and excitation up to 1700nm: imaging, photostimulation and electrophysiology in one experience
Product overview
- Ultima 2Pplus
- Bruker
- USA
- Ultima
- Ultima 2Pplus
FN 28mm and excitation up to 1700nm: imaging, photostimulation and electrophysiology in one experience
Fully Optical Multiphoton Workstation for In vivo Research
Multiphoton workstation for deep imaging, photostimulation and electrophysiology in a single experiment.
Two independent scanning paths allow both image capture and photoactivation to occur simultaneously, and the motorized sample positioning and modular architecture support intravital protocols, optogenetics and high-speed volumetric photography.
The FN 28 mm optical field exceeds the standard FN 18 mm; the optical path is designed to excite up to 1700 nm for three-photon tasks. Optional resonance scanner provides up to 30 fps at 512 x 512.
Key figures
1700 nm
three-photon
30 frames/s
512×512, resonance scanner
4
phEU detectors
Applications
- Neuroscience and optogenetics
- Intravital visualization
- Immunology and immuno-oncology
- Three-photon deep visualization
- Calcium imagery
- Electrophysiology in slices
Models and configurations
- Ultima 2Pplus
Product details
Ultima 2Pplus
increased table gap for large animals
Modular expansion
Photostimulation, FLIM, resonance scanner
More field and depth
- FN 28 mm – more than 50% larger than the standard FN 18 mm, while maintaining resolution to the edges
- The optics of the emission path with a diameter of 2 inches and detectors in the immediate vicinity collect a maximum of scattered photons.
- Optical input is designed for 690-1700 nm, including three-photon imaging
Simultaneous stimulation and shooting
- Second independent scanning path for photostimulation or ancading during imaging
- Holographic 3D Photo Stimulation with Neuralight 3D Modulator
- Electrically adjustable focusing allows you to move the plane of the shooting regardless of the plane of stimulation.
Platform flexibility
- Modules: resonance scanner, FLIM, two-wave imaging, LED optogenetics, rotary revolver head, Dodt contrast
- Prairie View with synchronization of external devices and electrophysiology
- Extended table gap for working with large animals
Scanning.
| Method of scanning | Cambridge 6mm pair of electroplating, raster and spiral scanning |
|---|---|
| Field of vision | ~1.375 × 1.375 mm with a 16× lens (FN ≤ 28 mm) |
| Speed (raster) | 1.65 fps at 512×512; >12 fps at 64 px. |
| Speed (spiral) | 6 fps at 512×512; ~30 fps at 64×64 |
| Resonance scanner (option) | 8 kHz; ~30 fps at 512×512; >1300 fps for ROI 512×8 |
| Scanning setup | 1–2048 pix/line and rows/frame; zoom to 120×; 360° rotation; linear and point scans |
| Photomanipulation (option) | a second set of 3 or 6 mm galvanometers in the same scanning head |
Detectors.
| The Reflected Channel (non-descanned) | 1-4 Hamamatsu Multi-Alkali, upgrade to GaAsP |
|---|---|
| The passing channel (non-descanned) | 1–2 Hamamatsu Multi-Alkali FEU, upgrade to GaAsP |
| Others. | FEU for contrast Dodt and passing light; C-mount camera port |
Optical inputs
| Multiphoton laser | 690-1700 nm |
|---|---|
| Epifluorescence | turret-powered |
| Visible lasers | fiber-entry |
| Laser safety class | Class 1 with a light-shielding casing |
Motorization
| XY-table | ~15 cm X, ~7.5 cm Y, step ~0.3 μm |
|---|---|
| XY-base displacement | ~35 mm, step 0.1 μm |
| Z-focus. | 30 mm, step ~0.2 μm |
| Z-pieze | up to 1000 μm, step 0.05 μm |
Software
| Software | Prairie View: Z- and T-series, Atlas montages, ROI, photoactivation, analog inputs/outputs for electrophysiology |
|---|
Expansion modules
Photostimulation Path Module
Simultaneous photostimulation or ankage during shooting
Neuralight 3D SLM
Holographic 3D Photo Stimulation of Groups of Cells
Resonant Scanner Module
High-speed shooting 30 frames / s at 512×512
Remote ETL Module
Optically corrected electrically adjustable lens, operating range of 450 μm
FLIM Module
Visualization of the lifetime of fluorescence and phosphorescence
Dual Wavelength Imaging Module
Introduce two lasers without rearrangement of wavelength
LED Module
Full-field optogenetics
Mechanics and detection
Rotating Nosepiece Module
Off-axis shooting, optionally motorized
Moving Scope / Moving Sample Stage Module
Moving a microscope or sample; Bridge Stage turns the system into a slicer
Objective Z-Piezo Stage Module
Fast volumetric shooting in synchronous with resonance scanner
Substage Detectors Module
Detection of weak signals in vitro and direct HVG (SHG)
Dodt Gradient Contrast Module
Contrast of unpainted slices in electrophysiology
Inverter Module
Translation to an inverted configuration
Related equipment types
- Femtosecond lasers for multiphoton microscopy
- Electrophysiology systems (patch-camp)
- Anti-vibration tables
- Incubation chambers for microscopes
Related products
- Thistle Scientific multiSUB — Incubation chambers for microscopes
Classification
- Immunology
- Multiphoton microscopes
- Microscopy and imaging systems
- Fluorescent microscopes and modules
- Ultima 2Pplus