300 Hz
MS/MS for targeted analysis
Micro Solutions / Life Science
TIMS-QTOF mass spectrometer timsMetabo
Desktop 4D metabolomics and lipidomics: separating isomers by CCS and MS/MS to 300 Hz
Product overview
- timsMetabo
- Bruker
- USA
- qTOF
- timsMetabo
Desktop 4D metabolomics and lipidomics: separating isomers by CCS and MS/MS to 300 Hz
TIMS-MX Analyzer, MoRE and Athena Ion Processor
TIMS-QTOF Desktop Platform for Highly Sensitive 4D Metabolomics and Lipidomics
timsMetabo combines TIMS-MX, Athena Ion Processor and VIP-HESI to separate isomers and isobars, obtain purified MS/MS spectra and confident annotation on m/z, retention time and CCS. One run simultaneously supports quantitative determination and structural characterization.
The system uses MoRE mode to extend the range of ion mobility, high-frequency electronics TIMS-MX and precision detection. In the official example for creatinine, the range is 22-22624 ng / ml; values depend on the method and matrix.
Key figures
CCS
4th dimension
< 5 %
RSD areas, 200+ injections
RUO
research
Applications
- Non-targeted metabolomics
- Lipidomy
- Search for biomarkers
- Separation of isomers
- PFAS and environmental pollutants
Models and configurations
- timsMetabo
Product details
timsMetabo
TIMS-QTOF desktop
4D annotation
m/z, retention time, CCS, MS/MS
Selectivity
- TIMS-MX Analyzer and MoRE Mode Expand M/Z Ranges and Mobility for Small Molecules
- Separation of isomers, isobars and interfering ions reduces chimeric MS/MS spectra
- Accurate and reproducible CCS values increase the confidence of the abstract
Sensitivity
- Athena Ion Processor boosts signal in MS and MS/MS modes
- VIP-HESI source for analytical flow rates; CSI for micro and nanoflow
- Creatinine is quantified in the range of 22–22,624 ng/ml
Quality control
- RSD area <5% and CCS deviation with standard deviation of 0.5% on 200+ urine injections
- QSee Test with Automatic “Yes/Not Good” Report
- TwinScape and TASQ RealTimeQC cloud monitoring software
Analyzer
| Type | TIMS-QTOF, desktop version |
|---|---|
| Ion mobility | Two-stage funnel TIMS-MX, More |
| quadrupole | High-precision, with Athena Ion Processor |
| MS/MS speed | up to 300 Hz (targeted quantitative analysis) |
| Collection regimes | PASEF, dia-PASEF |
Ionization and chromatography
| Sources | VIP-HESI, CaptiveSpray (CSI) |
|---|---|
| Chromatography | Analytical, Micro- and Nanoflow |
Indicators (producer data)
| Creatinine, Quantitative Definition Range | 22–22,624 ng/ml |
|---|---|
| Area reproducibility (NIST SRM 3672 urine) | RSD < 5%, more than 200 injections |
| Stability of CCS | Standard deviation ΔCCS 0.5% |
P.O. and status
| Processing | MetaboScape (CCS-Predict Pro, MetFrag, BioTransformer), TASQ |
|---|---|
| Purpose | RUO – for research purposes only |
Columns
Bio-AQ
C18 with polar functionality, 100% aqueous phase compatible for 4D metabolomics
Bio-LP
C8-phase for 4D lipidomics at 50% isopropanol and low pressure
Quality control
QSee 8-mix Performance Test
Testing the performance of LC-TIMS-MS before the experiment
TwinScape
Cloud-based monitoring of productivity trends
Spectrum libraries
MetaboBASE Personal Library 3.0
MS/MS spectra of more than 100,000 standards, in-silico spectra of more than 233,000 connections
Bruker HMDB Metabolite Library 2.0
More than 6,000 spectra from over 800 standards
Bruker NIST 2020 Mass Spectral Library
EI and MS/MS spectra, retention indices
MetaboBASE Plant Library
Spectra and CCS for plant metabolites
Related equipment types
- HPLC/UHPLC systems
- Nitrogen generators
- Centrifuges
- Freezers -80 °C
Related products
- Cence TG16-WS — Centrifuges
- Heal Force MPC5 — Freezers -80 °C
Classification
- Toxicology and omics
- Metabolomics
- Analytical instruments for biological samples
- Mass spectrometry
- Q-TOF and ionic mobility (timsTOF): proteomics, metabolomics
- TIMS-QTOF mass spectrometer timsMetabo