Bruker
Microscopes of the light sheet of the Luxendo MuVi SPIM and InVi SPIM series
From living embryos to enlightened mouse brains: multi-track and lattice light-sheet shooting
Luxendo’s line of light-leaf microscopes covers tasks from multi-day imaging of living embryos to imaging the enlightened mouse brain and subcellular structures.
Request a quotationMuVi SPIM illuminates and registers a sample from two opposite sides, supports 360° rotation and is available in LS versions for living and CS for enlightened samples. InVi SPIM Lattice Pro in inverted geometry forms a gauss, Bessel or lattice light sheet using a light modulator and supports SIM, FLIM and FCS. The systems can be equipped with a photo manipulation module and are controlled by LuxControl software.
MuVi SPIM: up to six laser lines 405–785 nm, two-color detection, control 20–37 °C, CO2/O2/N2 and humidity (LS), the volume of the illuminated sample up to 15 × 15 × 20 mm (CS), lenses 4×–20 × InVi SPIM Lattice Pro: 25× 1.1 NA detection lens, 310 nm XY and 530 nm Z in optical lattice mode, 80 frame / s at full frame and up to 500 frame / s on a reduced field.
MuVi SPIM: Multi-Perspective Shooting
- Simultaneous lighting and detection from two opposite sides without rotating the sample
- Free axis of rotation and isotropic resolution at one rotation by 90°
- Simultaneous two-color detection, up to six laser lines 405-785 nm
- Scanned beam and pivot scanning eliminate stripes and shadows
Living and enlightened patterns
- LS performance: temperature control 20–37 °C, CO2, O2, N2 and humidity for hours and days
- CS-performance is compatible with any methods of enlightenment, top-mounting reduces contact with reagents
- Transition between tasks – rapid change of lenses
InVi SPIM Lattice Pro and Software
- Inverted geometry and light modulator: gauss, Bessel, static sheet and optical lattice
- SIM, FLIM and FCS capabilities; up to 500 fps on reduced field
- LuxControl software: crosslinking hundreds of tiles, merging angles, deconvolution, 3D viewing of multiterabyte data
- Optional photo manipulation module for all three systems
Lens and field of view
| Parameter | MuVi SPIM LS | MuVi SPIM CS | InVi SPIM Lattice Pro |
|---|---|---|---|
| Objects of detection | 20× 1,0 NA; 16× 0,8 NA | 20× 1,0 NA; 10× 0,5 NA; 4× 0,28 NA | Nikon CFI Apo 25×W 1.1 NA with correction ring |
| Lighting lens | — | — | Special Optics 28.6×0.7 NA, water immersion |
| Effective scaling up | 8–44,4× | 2,2–40× | 31.3× or 62.5× |
| Pixel size | 146–812 nm | 163-2925 nm | 104 or 208 nm |
| Field of vision | 299-1664 μm | 333–5990 μm | 213 or 426 μm |
Resolution and speed
| Parameter | MuVi SPIM LS | MuVi SPIM CS | InVi SPIM Lattice Pro |
|---|---|---|---|
| Resolution XY/Z | — | — | 310 nm/530 nm (optical gratings); 680 nm (Bessel); 750 nm (gauss); 800 nm (static sheet) |
| Speed | — | — | 80 fps full frame; up to 500 fps on reduced field |
Lighting and detection
| Parameter | MuVi SPIM LS | MuVi SPIM CS | InVi SPIM Lattice Pro |
|---|---|---|---|
| Laser lines | up to 6, 405–785 nm | up to 6, 405–785 nm | 6 |
| Detection | simultaneous | simultaneous | 2 sCMOS cameras, up to 10 filters per camera |
| Geometry | 4 axes: 2 lighting + 2 detections, rotation 360° | bilateral lighting, single or bilateral detection | inverted |
Samples and environment
| Parameter | MuVi SPIM LS | MuVi SPIM CS | InVi SPIM Lattice Pro |
|---|---|---|---|
| Environmental control | 20–37 °C; CO2, O2, N2; humidity | — | 20–37 °C; CO2, O2, N2; humidity |
| Samples. | living organisms and embryos | organoids to the enlightened mouse brain; scanned volume 15 × 15 × 20 mm | Single cells, 3D cultures, small organisms |
| Installation | — | hook, plate, pin, cuvette; node 3D-movement / rotation from above | — |
Software
| Parameter | MuVi SPIM LS | MuVi SPIM CS | InVi SPIM Lattice Pro |
|---|---|---|---|
| Software | LuxControl: collection, viewing, processing; TIFF, HDF5, IMS formats; script management |