Bruker
Mass spectrometers with ion mobility of the timsTOF series
4D multi-multiomics with ion mobility separation: PASEF 120 to 300 Hz
The TIMS-QTOF series of mass spectrometers for 4D multiomics: ion mobility and CCS add another separation dimension to chromatography and precise mass.
Request a quotationThe timsTOF systems separate ions in the gas phase by the TIMS method and, using PASEF, sequentially fragment them, increasing the speed and depth of identification. The line covers the universal timsTOF Pro 2, high-performance timsTOF HT and ultrasensitive timsUltra AIP for small sample quantities.
PASEF speeds are more than 120 Hz for timsTOF Pro 2, more than 150 Hz for timsTOF HT and up to 300 Hz for timsUltra AIP. TIMS dual analyzer operates with a duty cycle of about 100%; PASEF, dia-PASEF and prm-PASEF modes are supported. timsTOF HT is equipped with a 14-bit digitizer, timsUltra AIP - Athena Ion Processor with more than two-fold signal gain.
TIMS and PASEF
- Double TIMS accumulates ions in the first section and sequentially releases them from the second – the duty cycle is close to 100%.
- PASEF synchronizes quadrupole insulation with ion output from TIMS and accelerates MS/MS
- Support for PASEF, dia-PASEF and prm-PASEF regimes
Depth and dynamic range
- timsTOF HT: 4th generation TIMS-XR analyzer and 14-bit digitizer
- More than 5 orders of relative dynamic range in dia-PASEF
- timsTOF Pro 2: triple the ion capacity of dual-TIMS
Limit sensitivity
- TIMSUltra AIP: Athena Ion Processor gives more than two times the signal increase
- PASEF up to 300 Hz and programmable mass transmission range
- More than 7,000 protein groups on average in a 5-minute gradient
Analyzer
| Parameter | timsTOF Pro 2 | timsTOF HT | timsUltra AIP |
|---|---|---|---|
| TIMS analyzer | Dual-TIMS, three times the ion capacity | TIMS-XR 4th Generation | Dual TIMS-XR + Athena Ion Processor |
| PASEF Speed | > 120 Hz | > 150 Hz | 300 Hz |
| TIMS work cycle | about 100% | ||
| digitizer | — | 14-bit (ADT) | — |
Regimes and ionization
| Parameter | timsTOF Pro 2 | timsTOF HT | timsUltra AIP |
|---|---|---|---|
| Collection regimes | PASEF, dia-PASEF, prm-PASEF | PASEF, dia-PASEF, prm-PASEF | All PASEF regimes, including diagonal-PASEF |
| Source | — | — | CaptiveSpray (CSI) with optimized gas flow |
| Features | CCS analysis for 4D multiomics | High Dynamic Range for High-Performance Proteomics | ICC 2.0; upgrades with timsTOF SCP, Ultra and Ultra 2 |
Software
| Parameter | timsTOF Pro 2 | timsTOF HT | timsUltra AIP |
|---|---|---|---|
| Processing | PaSER, MetaboScape, DIA-NN | PaSER, DIA-NN, Spectronaut | ProteoScape with Spectronaut 20 |