Micro Solutions / Life Science

Kino optical in vivo imaging system

Personal BLI/FLI imaging: up to three mice per shot on a laboratory table

Product overview

Kino
Bruker
USA
Optical in vivo visualization
Kino

Personal BLI/FLI imaging: up to three mice per shot on a laboratory table

Spectral Instruments Imaging compact system with LED excitation and Aura software

BLI/FLI personal desktop system for small research groups and longitudinal experiments.

Kino captures bioluminescent and fluorescent images of up to three mice, microplates and other samples, and a compact camera, heated platform, LED excitation and Aura software enable a quick transition from experiment to quantitative output.

Capacity - 3 mice, maximum field of view - 13×13 cm Supported 10 wavelengths of LED-excitation and 5 emission filters; CCD-camera has absolute solid-state cooling to -90 ° C. X-ray channel in Kino is not provided.

Key figures

3

mouse-to-cap

13×13 cm

field

−90 °C

air-cooling

BLI · FLI

two-modalities

Applications

Tumor models
Distribution of labeled compounds
Inflammation.
Multiplex NIR fluorescence
Visualization of microplates and samples

Models and configurations

Kino

Product details

Compact desktop system

56 × 66 × 122 cm

Quantitative results in Aura

calibration traceable to NIST

Sensitivity and quality of data

CCD-camera cooled to −90 °C air and Peltier elements - no liquids and chillers, ready for operation in about 5 minutes
Patented LED excitation without fiber: strong fluorescence signal at low background
Absolute calibration traceable to NIST keeps data comparability when changing camera settings
Minimum recorded brightness - 59 photons / s / cm2 / s

For small groups

Optimized sensor and field of view 13×13 cm designed to shoot up to 3 mice
Easy Mode and Smart AutoExp make shooting easier for new users
Kinetics Mode Automatically Builds Bioluminescence Kinetic Curves

Convenience and reliability

Heated platform (20–40 °C) and ports for gas anesthesia
Vertical door saves space and reduces the output of the anesthetic in the room
Steel light-tight housing without wearable seals, factory calibration
Aura analysis software for free for Windows and Mac, opens Living Image files

Modality and capacity

Visualization techniques	Bioluminescence (BLI), fluorescence (FLI)
X-ray	not provided
Capacity	up to 3 mice
Maximum optical field of view	13 × 13 cm
Visualization platform	50x34 cm, heated 20-40 °C
Gas anesthesia	port-in

Camera.

Sensor	CCD 0.5" with backlight, Grade 1 plus
Sensor resolution	1024 × 1024 pixels
Sensor size	13 x 13 mm
Cooling	-90 °C, solid state, air
Quantum efficiency	>85% in the 500–700 nm range; >35% in the 700–900 nm range
Dark current.	<100 e−/s/cm2
binning	1×1, 2×2, 4×4, 8×8
Objective	50 mm, aperture f/1.2–f/16
Minimum recorded brightness	59 photons/s/cm2/sp

fluorescence

LED-excitation	10 wavelengths: 430, 465, 500, 535, 570, 605, 640, 675, 710, 745 nm
Emission filters	5 filters to choose from 20 standard (490–870 nm)
LED resource	100,000 hrs

General.

Dimensions (E × G × B)	56 × 66 × 122 cm
Calibration	absolute, traceable to NIST; factory
Set	PC, monitor, Aura software

Options

Light-tight access port

Connecting external systems to the camera; installed at the site of operation

Additional emission filters

Replacement by an operator from a standard set of 490-870 nm, custom-made

Related equipment types

Gas anesthesia systems
Image analysis software
CO2 incubators

Classification

Scientific and production equipment
Visualization of small animals - optical
Microscopy and imaging systems
Fluorescent microscopes and modules
Kino