Micro Solutions / Life Science

Optical in vivo imaging system Ami HTX

Bioluminescence, fluorescence and X-rays for five mice in one exposure

Product overview

Ami HTX
Bruker
USA
Optical in vivo visualization
Ami HTX

Bioluminescence, fluorescence and X-rays for five mice in one exposure

Spectral Instruments Imaging desktop system with LED excitation and Aura software

Desktop BLI/FLI/X-ray system for high-performance imaging of up to five small animals.

Ami HTX combines bioluminescence, fluorescence and X-rays in a compact system with automatic field of view and filter selection. Aura performs calibrated quantification, and a heated platform and anesthesiological connections support longitudinal studies.

The system is designed for 5 mice; the maximum optical field of view is 25×17 cm The camera is cooled to −90 °C, 10 LED-excitation wavelengths and 10 emission filters are used; the HTX configuration includes an X-ray channel.

Key figures

5

rats

25×17 cm

optical field

−90 °C

air-cooling

BLI · FLI · X-ray

three-modalities

Applications

Tumor models
Distribution of labeled compounds
Inflammation.
Multiplex NIR fluorescence
Bioluminescence kinetics

Models and configurations

Ami HTX

Product details

Desktop system BLI/FLI/X-ray

56 × 66 × 122 cm

Quantitative results in Aura

calibration traceable to NIST

Sensitivity and quality of data

Camera with CCD sensor cools to -90 °C due to Peltier elements and air - no liquids, chillers and risk of leaks, ready for operation in about 5 minutes
The patented LED excitation delivers significantly more light to the object than a white light bulb, without fiber optic – a higher signal and below the background
The absolute calibration traceable to the NIST standard makes the data comparable between the series and the camera settings.
Minimum recorded brightness - 50 photons / s / cm2 / s

Throughput

25x17 cm field of view allows you to shoot 5 mice at the same time
Automatic X-ray channel: anatomical binding without animal relaying
Easy Mode: Select probe and object and start shooting; Smart AutoExp selects exposure automatically
Kinetics Mode Automatically Builds Bioluminescence Kinetic Curves in Real Time

Convenience and reliability

Heated platform (20–40°C) and gas anesthesia ports are compatible with third-party systems
Vertical door saves space and reduces the output of the anesthetic in the room
Lightproof steel case without seals and end switches, factory calibration without installation on site
Aura analysis software is free for Windows and Mac and opens Living Image files

Modality and capacity

Visualization techniques	bioluminescence (BLI), fluorescence (FLI), X-ray
Capacity	up to 5 mice
Optical field of view	10×7 to 25×17 cm
Visualization platform	50x34 cm, heated 20-40 °C
Gas anesthesia	port-in

Camera.

Sensor	CCD 1" with backlight, Grade 1 plus
Sensor resolution	1152 × 770 pixels
Sensor size	25.9 x 17.3 mm
Cooling	-90 °C, solid state, air
Quantum efficiency	>85% in the 500–700 nm range; >35% in the 700–900 nm range
Dark current.	<49 e−/s/cm2
binning	1×1, 2×2, 4×4, 8×8
Objective	50 mm, aperture f/1.2–f/16
Minimum recorded brightness	50 photons/s/cm2/sp

fluorescence

LED-excitation	10 wavelengths: 430, 465, 500, 535, 570, 605, 640, 675, 710, 745 nm
Emission filters	10 filters to choose from 20 standard (490-870 nm, step 20 nm)
LED resource	100,000 hrs

X-ray

Source	10–40 keV
Detector	linear scanning
Field of vision	25 x 15 cm

General.

Dimensions (E × G × B)	56 × 66 × 122 cm
Calibration	absolute, traceable to NIST; factory
Set	PC, monitor, Aura software
Software	Aura (collection and analysis); analysis – unlicensed, Windows and Mac

Options

Additional emission filters

Replacement of filters by the operator from a set of standard 490-870 nm, custom-made

Related equipment types

Gas anesthesia systems
Micro-CT
Image analysis software
Laminar flow cabinets

Classification

Scientific and production equipment
Visualization of small animals - optical
Microscopy and imaging systems
Fluorescent microscopes and modules
Ami HTX