Micro Solutions / Life Science

Microscopes of the light sheet of the Luxendo MuVi SPIM and InVi SPIM series

From living embryos to enlightened mouse brains: multi-track and lattice light-sheet shooting

Product overview

MuVi and InVi range
Bruker (Luxendo)
Germany
Luxendo
MuVi and InVi range

From living embryos to enlightened mouse brains: multi-track and lattice light-sheet shooting

MuVi SPIM LS and CS for living and enlightened specimens, InVi SPIM Lattice Pro

Luxendo’s line of light-leaf microscopes covers tasks from multi-day imaging of living embryos to imaging the enlightened mouse brain and subcellular structures.

MuVi SPIM illuminates and registers a sample from two opposite sides, supports 360° rotation and is available in LS versions for living and CS for enlightened samples. InVi SPIM Lattice Pro in inverted geometry forms a gauss, Bessel or lattice light sheet using a light modulator and supports SIM, FLIM and FCS. The systems can be equipped with a photo manipulation module and are controlled by LuxControl software.

MuVi SPIM: up to six laser lines 405–785 nm, two-color detection, control 20–37 °C, CO2/O2/N2 and humidity (LS), the volume of the illuminated sample up to 15 × 15 × 20 mm (CS), lenses 4×–20 × InVi SPIM Lattice Pro: 25× 1.1 NA detection lens, 310 nm XY and 530 nm Z in optical lattice mode, 80 frame / s at full frame and up to 500 frame / s on a reduced field.

Key figures

360°

multi-view

6

laser-line

15×15×20 mm

volume

20–37 °C

environment

Applications

Development biology
Neurobiology
Oncology and Tumorogenesis
Enlightened organs and brain
Single cells and 3D cultures
Photomanipulation and FRAP

Models and configurations

MuVi SPIM LS
MuVi SPIM CS
InVi SPIM Lattice Pro

Product details

MuVi SPIM

4-axis circuit: two lighting lenses and two detections

Enlightened samples

CS module is compatible with any enlightenment environment

MuVi SPIM: Multi-Perspective Shooting

Simultaneous lighting and detection from two opposite sides without rotating the sample
Free axis of rotation and isotropic resolution at one rotation by 90°
Simultaneous two-color detection, up to six laser lines 405-785 nm
Scanned beam and pivot scanning eliminate stripes and shadows

Living and enlightened patterns

LS performance: temperature control 20–37 °C, CO2, O2, N2 and humidity for hours and days
CS-performance is compatible with any methods of enlightenment, top-mounting reduces contact with reagents
Transition between tasks – rapid change of lenses

InVi SPIM Lattice Pro and Software

Inverted geometry and light modulator: gauss, Bessel, static sheet and optical lattice
SIM, FLIM and FCS capabilities; up to 500 fps on reduced field
LuxControl software: crosslinking hundreds of tiles, merging angles, deconvolution, 3D viewing of multiterabyte data
Optional photo manipulation module for all three systems

Lens and field of view

Objects of detection	20× 1,0 NA; 16× 0,8 NA 20× 1,0 NA; 10× 0,5 NA; 4× 0,28 NA Nikon CFI Apo 25×W 1.1 NA with correction ring
Lighting lens	— — Special Optics 28.6×0.7 NA, water immersion
Effective scaling up	8–44,4× 2,2–40× 31.3× or 62.5×
Pixel size	146–812 nm 163-2925 nm 104 or 208 nm
Field of vision	299-1664 μm 333–5990 μm 213 or 426 μm

Resolution and speed

Resolution XY/Z	— — 310 nm/530 nm (optical gratings); 680 nm (Bessel); 750 nm (gauss); 800 nm (static sheet)
Speed	— — 80 fps full frame; up to 500 fps on reduced field

Lighting and detection

Laser lines	up to 6, 405–785 nm up to 6, 405–785 nm 6
Detection	simultaneous simultaneous 2 sCMOS cameras, up to 10 filters per camera
Geometry	4 axes: 2 lighting + 2 detections, rotation 360° bilateral lighting, single or bilateral detection inverted

Samples and environment

Environmental control	20–37 °C; CO2, O2, N2; humidity — 20–37 °C; CO2, O2, N2; humidity
Samples.	living organisms and embryos organoids to the enlightened mouse brain; scanned volume 15 × 15 × 20 mm Single cells, 3D cultures, small organisms
Installation	— hook, plate, pin, cuvette; node 3D-movement / rotation from above —

Software

Software	LuxControl: collection, viewing, processing; TIFF, HDF5, IMS formats; script management

Options

Photomanipulation Module

Photoablation, optogenetics, FRAP; ROI point/line/arbitrary/square; lasers from CW UV to pulsed IR

Environmental control module

Temperatures 20-37 °C, CO2, O2, N2, humidity

Advanced Illumination Module (AIM)

Lattice lighting to improve resolution in 3D cultures

Related equipment types

CO2 incubators
Biosafety cabinets
Workstations for image analysis
Water treatment systems

Classification