BGI
DNBSEQ Library Preparation and Sequencing Kits
MGIEasy’s single line of reagents for library preparation and sequencing on DNBSEQ platforms
BGI DNBSEQ sets form a consistent set of reagents for library preparation, cyclization, DNB production and sequencing on platforms of different performance.
Request a quotationThe range covers PCR-free and enzymatic fragmentation, WGS/WES, RNA and small RNA, methylation, targeted panels, adapters and magnetic particles. The sets are selected by sample type, application, sequencer and required reading length.
The formats are available for E25, G99, G400, T1+, T7 and other DNBSEQ platforms; single- and dual-index adapters, DNB Make sets and modular sequencing sets are used.
Sets for WGS and WES
- WGS: MGIEasy Fast FS, Universal DNA, PCR-Free DNA, UDB Universal and Duplex UMI Universal Library Prep Set
- WES: MGIEasy Exome Capture V5 Probe Set, Fast Hybridization and Wash Kit, Exome Capture Accessory Kit and Dual Barcode Exome Capture Accessory Kit
RNA, methylation and special tasks
- RNA: MGIEasy Fast RNA Library Prep Set, rRNA Depletion Kit, rRNA & Globin Depletion Kit, Small RNA Library Prep Kit
- Methylation: MGIEasy Whole Genome Methylation Sequencing Library Prep Kit
- cfDNA and forensic genetics: Cell-free DNA Library Prep Set, Pa-SNPs Genotyping Kit, Signature Identification Library Prep Kit
- ATOPlex series for targeted panels: respiratory pathogens, MTB, HIV-1, metagenomic DNA panels (16S/18S/ITS1 and others)
Cycling, Barcoding and DNB Obtaining
- MGIEasy Circularization Kit and Dual Barcode Circularization Kit
- Adapters and Barcodes: MGIEasy DNA Adapters-96 (Plate) Kit, UDB Primers Adapter Kit A/B/C
- DNBSEQ OneStep DNB Make Reagent Kit versions V1.0–V4.0, including single and dual barcode variants
Compatibility
| Parameter | MGIEasy / DNBSEQ |
|---|---|
| DNBSEQ sequencing | E25, G99, G400, T1+, T7 and other DNBSEQ platforms |
| Types of specimens | fragmented and whole gDNA, FFPE DNA, Meta DNA, cfDNA, ChIP DNA |
Methods of training
| Parameter | MGIEasy / DNBSEQ |
|---|---|
| Fragmentation | enzymatic or using Covaris |
| Amplification | PCR-based or PCR-free method |
| Barcoding | Single and Dual-Index Adapters (UDB) |