<6 amol
ATP/well, luminescence
Micro Solutions / Life Science
Multimodal TriStar 5 Reader
Modular reader with independent monochromators and filters for complex multimodal tasks
Product overview
- TriStar 5
- Berthold
- Germany
- TriStar
- TriStar 5
Modular reader with independent monochromators and filters for complex multimodal tasks
FlexTec optics, HTRF/TR-FRET support and up to 3 JET injectors
TriStar 5 is a modular high-performance multimodal reader for complex immunological, cellular and molecular biology studies with a flexible selection of filters and monochromators.
The platform combines absorption, fluorescence, luminescence, TRF, TR-FRET/HTRF and fluorescence polarization; depending on the configuration, BRET, NanoBRET, LanthaScreen and AlphaScreen are available. FlexTec allows you to combine filters and monochromators, upper and lower reading supports both solute and cellular assays, and optional JET injectors are designed for rapid kinetics.
Source of excitation - xenon pulse lamp 200-1000 nm. FEU operates in the range of 280-650 nm, optionally up to 850 nm; photodiode - 200-1000 nm Double monochromators have a variable band 4-12 nm on excitation and 8-22 nm on emission in 1 nm increments Sensitivity: less than 6 amol ATP/well for luminescence, less than 7 amol FITC/well for Fluorescence and less than 5 amol/well for TRF. Microplates 6-384 wells; up to three injectors 10-100 μl, 200-440 μl / s, accuracy and precision better than 2% Incubation up to 45 ° C; dimensions 391×470×395 mm, weight about 32 kg.
Key figures
4-22 nm
monochromator
≤384
hole
RUO
research
Applications
- HTRF and TR-FRET assays
- Fluorescent polarization (FP)
- Time-resolved fluorescence (TRF)
- AlphaScreen/AlphaLISA
- Cellular and biochemical analyses
Models and configurations
- Tristar 5
Product details
Modular multimodal reader
top-reading
FlexTec optics
filters and monochromators in one system
FlexTec optics
- Independent twin monochromators on excitation and emission with variable bandwidths of 4-12 nm and 8-22 nm, step 1 nm
- Three optical paths to choose from: filter + filter, monochromator + monochromator, monochromator + filter
- Suppression of parasitic light to 10-6
Measurement technologies
- Supports HTRF, TR-FRET, TRF, fluorescent polarization, AlphaScreen/AlphaLISA, BRET/BRET2/NanoBRET, LanthaScreen
- Top and bottom reading of the microplate - for cellular and solution analyses
- Up to 3 JET Injectors for Flash Kinetics
ergonomics
- Full frontal access to microplate, filters and reagents
- Built-in refrigerated compartment for reagents
- Thermostating up to 45 °C and 3 stirring modes
Detector
| Detector | FEU in double mode 280-650 nm (opts. up to 850 nm) + photodiode 200-1000 nm |
|---|---|
| source of excitement | xenon pulse lamp, 200–1000 nm |
| Monochrome | 2 double, band 4-12 nm (excitation) and 8-22 nm (emission), step 1 nm |
Sensitivity and range
| luminescence | <6 amol ATP/well (96 holes) |
|---|---|
| fluorescence | <7 FITC amol/well (384 sv) |
| TRF | <5 amol/well |
| Absorption | accuracy better than 2%, precision better than 0.6% |
| Dynamic Range / Cross-talk | 6 orders (photon count), 0-3.5 OD (photodiode); cross-talk 10-6 |
Injectors and microplates
| Injectors | up to 3, JET technology, 10–100 μl, 200–440 μl/s, accuracy and precision better than 2% |
|---|---|
| Microplate formats | 6-384 holes, petri plates 35/60 mm, μDrop microplate, standard cuvettes |
General parameters
| Temperature control | +5 °C above room to 45 °C |
|---|---|
| Interface/PO | USB; Windows 10 (32/64 bit) |
| Dimensions (W×D×H) | 391×470×395 mm |
| Weight | about 32 kg |
| Certification | CE, NRTL |
Technology modules
Measurement technology TRF / TR-FRET-HTRF
Time-allowed fluorescence and HTRF module
Measurement technology FP
Fluorescent polarization module (fluorescein or TAMRA/Cy3)
Measurement technology LanthaScreen
LanthaScreen Analysis Module
Measurement technology AlphaScreen
AlphaScreen Analysis Module
JET Injector #1–#3
Additional JET Injectors
Facilities and consumables
LightCompass Basic / Professional / Plus
Data Collection and Analysis Software; Professional and Plus – FDA-supported 21 CFR Part 11
LB 9515 / LB 9516 test microplates
Validation of luminescent and absorption channels
μDrop Microvolume Plate
Microplate for small sample volumes
Cleanit Daily
Solution for cleaning injectors
Related equipment types
- Micro-plate wochers
- ELISA mini-stations
- Microvolume spectrophotometers
Related products
- DLab Scientific D3024 — Micro-plate wochers
- Micro Digital Nabi Plus — Microvolume spectrophotometers
Classification
- Clinical laboratory diagnostics
- Immunology
- ELISA analyzers
- Multimodal microplate readers
- Analytical instruments for biological samples
- Multimodal microplate readers
- TriStar 5