<6 amol
ATP/well, luminescence
Micro Solutions / Life Science
TriStar 3 Multimodal Reader
Multimodal filter reader: absorption, fluorescence and luminescence on one platform
Product overview
- TriStar 3
- Berthold
- Germany
- TriStar
- TriStar 3
Multimodal filter reader: absorption, fluorescence and luminescence on one platform
with ONE-4-ALL optics and optional JET injectors for fast kinetics
TriStar 3 is a sensitive filter multimodal reader for routine and research analysis, combining absorption, fluorescence and luminescence on one platform.
The device supports ELISA, DNA/RNA and protein quantification, cell tests, reporter assays, FRET and optional BRET/BRET2/NanoBRET. ONE-4-ALL optics and interchangeable filters are oriented to high sensitivity, and up to three optional JET injectors allow you to measure rapid kinetic reactions immediately after application. reagent.
Detection: photodiode 200-1000 nm for absorption and low-noise FEU 280-650 nm, optionally up to 850 nm Luminescence sensitivity - less than 6 amol ATP / well; fluorescence - less than 7 amol FITC / well; absorption 0-3.5 OD with an accuracy of better than 2% and precision better than 0.6 % Dynamic range of luminescence - 6 orders of magnitude, cross-talk to 10-6 for 6-384 well formats, also 35/60 mm dishes, cuvettes and microplates μDrop. Incubation up to 45 °C, three stirring modes; dimensions 391×470×344 mm, weight about 20 kg.
Key figures
≤384
hole
3
JET injectors
RUO
research
Applications
- Enzyme-linked immunosorbent assays (ELISA)
- Quantitative determination of DNA/RNA and protein
- Cellular and reporter analysis
- Protein-protein interactions (FRET, BRET)
Models and configurations
- Tristar 3
Product details
Desktop multimodal reader
frontal access to all nodes
Quick-replacement filters
Up to 40 excitation and emission filters
Detection
- ONE-4-ALL proprietary optics delivers equally high performance across all measurement modes
- Luminescence – less than 6 amol ATP/well; absorption – accuracy better than 2% and precision better than 0.6%
- Fast-shift filter sliders can accommodate up to 40 excitation and emission filters
Flexibility of application
- Supports ELISA, DNA/RNA and protein quantification, cellular and reporter tests, FRET
- Upgrade to BRET/BRET2 and NanoBRET
- Up to 3 Teflon JET injectors for fast kinetics and gentle supply of the reagent to living cells
ergonomics
- Full frontal access to microplate loading, filter change and reagent connection
- Built-in compartment for bottles with reagents and removable pallet for ice or water
- Flat top panel - a place for a laptop next to the device
Detector
| Detector | FEU in double mode 280-650 nm (opts. up to 850 nm) + photodiode 200-1000 nm |
|---|---|
| source of excitement | xenon pulse lamp, 200–1000 nm |
| Choosing a wavelength | interference-filter |
Sensitivity and range
| luminescence | <6 amol ATP/well (96 holes) |
|---|---|
| fluorescence | <7 FITC amol/well (384 sv) |
| Absorption | accuracy better than 2%, precision better than 0.6% |
| Dynamic range | 6 orders (photon count); 0–3.5 OD (photodiode) |
| Cross-talk | 10−6 (black plates) |
Injectors and microplates
| Injectors | up to 3, JET technology, 10–100 μl, 200–440 μl/s, accuracy and precision better than 2% |
|---|---|
| Microplate formats | 6-384 holes, petri dishes 35/60 mm, μDrop microplate (from 2 μl), standard cuvettes |
| Stirring | 3 modes of operation |
General parameters
| Temperature control | +5 °C above room to 45 °C |
|---|---|
| Interface/PO | USB; Windows 10 (32/64 bit) |
| Dimensions (W×D×H) | 391×470×344 mm |
| Weight | about 20 kg |
| Certification | CE, NRTL |
Injectors and detection modules
JET Injector #1 / #2 / #3
Additional ultrafast JET injectors for precision injection of reagent
BRET/BRET2 Package, BRET High Efficiency, BRET2 High Efficiency
BRET/BRET2 measurement modules
nanoBRET Package
NanoBRET measurement module
Chroma-Glo Package
Module for multiplex luminescent assays Chroma-Glo
Facilities and consumables
LightCompass Basic / Professional / Plus
Data Collection and Analysis Software; Professional and Plus – FDA-supported 21 CFR Part 11
μDrop Microvolume Plate
Microplate for measuring samples from 2 μl
LB 9515 / LB 9516 test microplates
Quality control of luminescent and absorption channels
Luminescence Performance Kit
Testing of fluorescent channel performance
Reagent filter set
Filter kit for reagents, 10 pcs.
Cleanit Daily
Solution for cleaning injectors, 2×250 ml
Related equipment types
- Micro-plate wochers
- ELISA mini-stations
- Microvolume spectrophotometers
Related products
- DLab Scientific D3024 — Micro-plate wochers
- Micro Digital Nabi Plus — Microvolume spectrophotometers
Classification
- Clinical laboratory diagnostics
- Immunology
- ELISA analyzers
- Multimodal microplate readers
- Analytical instruments for biological samples
- Multimodal microplate readers
- TriStar 3