600
Exposure to circadian rhythms
Micro Solutions / Life Science
Lifetime plant imaging system NightSHADE LB 985
Luminescent and fluorescent imaging of entire plants of the previous generation
Product overview
- NightSHADE LB 985
- Berthold
- Germany
- NightSHADE
- NightSHADE LB 985
Luminescent and fluorescent imaging of entire plants of the previous generation
Out of production; current platform - NightSHADE evo LB 985N
NightSHADE LB 985 is a previous generation in vivo imaging system for recording fluorescent and fluorescent signals in tissues, sprouts and entire plants.
Berthold’s official application notes document the use of LB 985 for long-term circadian rhythm studies, fluorescent reporter imaging, and trans-illumination experiments on GFP-containing samples. A light-tight cabinet, a coolable CCD camera, and the ability to conduct non-invasive, overhead or side-sighting. So we're going to take pictures of petri dishes and vertical plants, and lighting, temperature and humidity conditions can be shaped by a long experiment, and we're going to analyze the images in IndiGO software.
The official materials for the NightSHADE LB 985 configuration indicate cooled CCD camera, upper or lateral camera position, fluorescent and fluorescent modes. The circadian rhythm application note describes measurements with the upper CCD camera, 600 c exposure and 1x1 binning; fluorescence note - excitation / emission filters 475/520 nm and For the IkLu configuration, the 365 and 470 nm trans illuminators with intensity adjustment are documented. A full summary of the specifications and completeness will be added only after receiving a separate official brochure, namely the LB 985; the LB 985N parameters are not carried over to this card.
Key figures
475/520 nm
Excitation/emission filters (fluorescence)
365/470 nm
Transilluminators (IkLu configuration)
Applications
- Study of the circadian rhythms of plants
- Visualization of reporter genes of luminescence
- Control of GFP expression by transillumination
- Evaluation of stress reactions of plants
Models and configurations
- NightSHADE LB 985
Product details
NightShade LB 985
Image from the official application note Berthold
Confirmed applications
- Cooled CCD camera in the upper or lateral position - for shooting Petri cups and whole plants
- Measurements of circadian rhythms are made with an upper chamber, 600 c exposure and 1x1 binning.
- Fluorescent measurements are performed with excitation/emission filters of 475/520 nm in IndiGO software
IkLu configuration
- 365 and 470 nm transilluminators with intensity adjustment from 0 to 100% in increments of 10%
- 520/10 nm emission interference filter for GFP recording without reflections from plastic dish
- Suitable for sprouts in Petri dishes of different sizes
Confirmed measurement regimes
| Camera. | cooled CCD, upper or lateral position |
|---|---|
| Regimes | luminescence, fluorescence |
| Exposition (circadian rhythms) | 600s, 1x1 binning |
| Filters (fluorescence) | Excitation 475 nm/emission 520 nm |
| Transilluminators (IkLu configuration) | 365 and 470 nm, adjustment 0–100% |
| Software | IndiGO |
Related equipment types
- Multimodal microplate readers
- Test tube phosphate
Related products
- DLab Scientific D3024 — Multimodal microplate readers
Classification
- Scientific and production equipment
- Visualization of plant growth and physiology
- Analytical instruments for biological samples
- Molecular spectroscopy
- Fluorimeters and luminometers
- NightSHADE LB 985
Product images
Documents
Manufacturer sources
- Improved Experimental Setup for Analysis of Circadian Rhythms Using the NightSHADE LB 985
- Trans-illumination for GFP Expressing Plants Using NightSHADE LB 985 IkLu
- Detection of Fluorescence in Transgenic Arabidopsis Lines Using NightSHADE LB 985
- Current NightSHADE evo Product Family Page
- Berthold In Vivo Imaging Systems