Micro Solutions / Life Science

Reagent kits for MassARRAY: iPLEX, UltraSEEK, EpiTYPER

MassARRAY chemistry set for genotyping from routine variants to mutations with a 0.1% share

Product overview

Agena Bioscience
USA
iPLEX / UltraSEEK / EpiTYPER
iPLEX Pro, iPLEX HS, UltraSEEK, EpiTYPER

MassARRAY chemistry set for genotyping from routine variants to mutations with a 0.1% share

Choice of chemistry by type of sample, panel and required sensitivity

MassARRAY reagent kits allow you to customize the platform for a wide range of tasks - from routine genotyping of inherited variants to highly sensitive search for somatic mutations and quantitative analysis of methylation.

iPLEX Pro is designed for multiplex analysis of germinal variants; iPLEX HS increases sensitivity for somatic variants with low allelic lobe; UltraSEEK uses mutant allele enrichment for even more sensitive analysis; EpiTYPER/MassCLEAVE is used after bisulfite conversion for quantitative profiling. Methylation: The reagent sets are presented as a single compatible ruler, and the specific set is selected by sample type, panel type and sensitivity required.

iPLEX Pro supports up to 40-plex analysis and is commonly used for variants with an allelic fraction of 5-10% or higher. iPLEX HS is designed to detect from about 1% VAF, and UltraSEEK - up to 0.1% VAF under validated conditions. The actual detection limit depends on the matrix, quality and quantity of DNA, panel composition and analytical validation. Agena ClearSEEK reagents are also available for current projects; these are included after confirming a compatible panel and an up-to-date catalog number.

Key figures

5–10%

Sensitivity of iPLEX Pro

≥1%

sensitivity of iPLEX HS

≥0,1%

sensitivity of UltraSEEK

Applications

Geminal genotyping
Somatic and low-frequency options
Liquid biopsy
Methylation profiling (EpiTYPER)
Pharmacogenetics
GMO screening and breeding livestock

Models and configurations

iPLEX Pro
iPLEX HS
UltraSEEK
EpiTYPER (MassCLEAVE)

Product details

MassARRAY reagent kits

chemistry

PCR phase

amplification and prolongation of the primer

MALDI-TOF Detection

determination of the mass of the reaction

iPLEX Pro

Multiplex up to 40 variants in a reaction; the reaction contains all four nucleotides in equal concentration
For germinal genotyping, carrier screening, biomarker validation, pharmacogenetics, breeding animal husbandry and strain validation
Sensitivity 5-10% in the allelic lobe of the variant

iPLEX HS and UltraSEEK

iPLEX HS uses nucleotides in a modified ratio to mutant allele enrichment: sensitivity ≥1%, up to 15 markers in the reaction
UltraSEEK detects only a rare/mutant nucleotide: sensitivity ≥0.1%, up to 15 markers in the reaction
Samples: heterogeneous solid tumors, fine needle aspiration, circulating tumor DNA from plasma

EpiTYPER / MassCLEAVE

Quantitative analysis of CpG methylation after bisulfite conversion of the sample
Sensitivity to detect methylation changes - from 5%

Sensitivity and multiplex

Limit of detection	5–10% ≥1% ≥0,1% 5 % (change in methylation)
Markers for reactions	40 15 15 —
Principle of reaction	all 4 nucleotides in equal concentration nucleotides in modified ratio, enrichment of mutant alleles only rare/mutant nucleotide Bisulfite conversion + MassCLEAVE cleavage

Scope of application

Types of specimens	blood, serum, buccal cells, microdissected and freshly frozen tumor cells, hair follicles, WGA DNA heterogeneous solid tumors, fine needle aspiration, seed mixtures circulating tumor and extracellular DNA from plasma —
Typical tasks	pharmacogenetics, hereditary diseases, validation of biomarkers, breeding, validation of culture strains somatic mutations, low-frequency variants, validation of NGS findings, screening for GMOs oncogenic markers of resistance and progression, non-invasive testing quantification

Related equipment types

MassARRAY genotyping system
Bisulfite DNA conversion kits
Next-generation sequencing

Classification